6792期

最新文章

• A village injury, a lifelong mission AI翻译
  作者：Luo, Renqi
  作者单位：Huazhong Agricultural University
• 'Morning sickness hormone' may help reduce alcohol intake AI翻译
  作者：Offord, Catherine
• First science from private Moon lander challenges picture of lunar volcanism AI翻译
  作者：Voosen, Paul
• A global methane observation system to track climate feedbacks for verifiable climate impact AI翻译
  作者：Watts, Jennifer D.; Ordway, Elsa; Malone, Sparkle L.; Zhu, Qing; Palmer, Paul I.; Patel-Tupper, Dhruv; Ciais, Philippe; Li, Fa; Monteverde, Danielle R.; Arndt, Kyle A.; Bruhwiler, Lori; Buma, Brian; Cadillo-Quiroz, Hinsby; Euskirchen, Eugenie; Hoyt, Alison M.; Holgerson, Meredith; Hugelius, Gustaf; Jackson, Robert B.; Jacob, Daniel; Kuhn, Mckenzie; Natali, Susan M.; Peng, Shushi; Perryman, Clarice R.; Poulter, Benjamin; Rey-Sanchez, Camilo; Sagang, Le Bienfaiteur; Schuur, Edward A. G.; Varner, Ruth K.; Vargas, Rodrigo
  作者单位：University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; Yale University; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; University of Edinburgh; Universite Paris Saclay; University of Texas System; University of Texas Austin; National Oceanic Atmospheric Admin (NOAA) - USA; Environmental Defense Fund; Arizona State University; Arizona State University-Tempe; University of Alaska System; University of Alaska Fairbanks; University of Alaska System; University of Alaska Fairbanks; Stanford University; Cornell University; Stockholm University; Harvard University; University of British Columbia; Peking University; North Carolina State University; Northern Arizona University; University System Of New Hampshire; University of New Hampshire; Stockholm University
• Thalamic activation of the visual cortex at the single-synapse level AI翻译
  作者：Chen, Yang; Kloos, Marinus; Varga, Zsuzsanna; Zhang, Yonghai; Piro, Inken; Sato, Tatsuo K.; Sakmann, Bert; Nelken, Israel; Konnerth, Arthur
  作者单位：Technical University of Munich; Kagoshima University; Hebrew University of Jerusalem; Hebrew University of Jerusalem
  摘要：Deciphering thalamocortical (TC) activation at the level of individual synapses is essential to understanding how the cortex processes sensory information. In this work, we studied TC computation underlying the emergence of orientation selectivity in the mammalian primary visual cortex (V1). Using two-photon glutamate imaging and optogenetic cortical silencing in vivo, we identified and characterized TC synapses onto mouse V1 layer 4 neurons. We found that TC- but not corticocortical-recipient...
• Chromatin buffers torsional stress during transcription AI翻译
  作者：Qian, Jin; Lubkowska, Lucyna; Zhang, Shuming; Tan, Chuang; Hong, Yifeng; Jia, Xiaomeng; Fulbright, Robert M.; Inman, James T.; Kay, Taryn M.; Jeong, Joshua; Hauk, Glenn; Gotte, Deanna; Berger, James M.; Kashlev, Mikhail; Wang, Michelle D.
  作者单位：Cornell University; Cornell University; Cornell University; Howard Hughes Medical Institute; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Cornell University; Cornell University; Johns Hopkins University; Johns Hopkins Medicine; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
  摘要：During eukaryotic transcription, RNA polymerase II (Pol II) must overcome nucleosome obstacles and, because of DNA's helical structure, must also rotate relative to DNA, which generates torsional stress. However, there is limited understanding of how Pol II transcribes through nucleosomes while supercoiling DNA. In this work, we determined that Pol II generates a torque of 9 piconewton-nanometers (pN & centerdot;nm) alone and 13 pN & centerdot;nm with transcription factor IIS (TFIIS), making i...
• Structure and organization of AMPA receptor-TARP complexes in the mammalian cerebellum AI翻译
  作者：Scrutton, Alexander M.; Sengupta, Nayanika; Ivica, Josip; Stockwell, Imogen; Peak-Chew, Sew; Singh, Bishal; Suzuki, Kunimichi; Chang, Veronica T.; McLaughlin, Stephen H.; Krieger, James M.; Aricescu, A. Radu; Greger, Ingo H.
  作者单位：MRC Laboratory Molecular Biology; UK Research & Innovation (UKRI); Medical Research Council UK (MRC); MRC Laboratory Molecular Biology; UK Research & Innovation (UKRI); Medical Research Council UK (MRC); University of Texas System; UTMD Anderson Cancer Center; Keio University; MRC Laboratory Molecular Biology; UK Research & Innovation (UKRI); Medical Research Council UK (MRC)
  摘要：AMPA receptors (AMPARs) are multimodal transducers of glutamatergic signals throughout the brain. Their diversity is exemplified in the cerebellum: At afferent synapses, AMPARs mediate high-frequency excitation, whereas in Bergmann glia (BG) they support calcium transients that modulate synaptic transmission. This spectrum arises from different combinations of core subunits (GluA1-4), auxiliary proteins, and posttranscriptional modifications. Using mass spectrometry, cryo-electron microscopy, ...
• A genetically encoded device for transcriptome storage in mammalian cells AI翻译
  作者：Chao, Yu-Kai; Wu, Michelle; Gong, Qiyu; Chen, Fei
  作者单位：Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Harvard University
  摘要：Understanding how cells make decisions over time requires the ability to link past molecular states to future phenotypic outcomes. We present TimeVault, a genetically encoded system that records and stores transcriptomes within living mammalian cells for future readout. TimeVault leverages engineered vault particles that capture messenger RNA through polyadenosine [poly (A)]-binding protein. We demonstrate that the transcriptome stored by TimeVaults is stable in living cells for more than 7 da...
• An out of this world buddy movie AI翻译
  作者：Faherty, Jackie
  作者单位：American Museum of Natural History (AMNH)