6813期

最新文章

• A cinnamyl alcohol dehydrogenase-like scaffold organizes monoterpenoid indole alkaloid biosynthesis AI翻译
  作者：Gao, Di; Mann, Scott Galeung Alexander; Chen, Binbin; Gou, Yuanwei; Chen, Cong; Qiao, Chong; Garza-Garcia, Jorge Jonathan Oswaldo; Shahsavarani, Mohammadamin; Jiang, Xiaojing; Tran, Hannah Caroline; Bao, Jingfei; Richardson, Mathew Bailey; Li, Jianing; Perley, Jacob Owen; Hwang, Jaewook; Dong, Feng; Dong, Chang; Huang, Lei; De Luca, Vincenzo; Wang, Yajie; Qu, Yang; Lian, Jiazhang
  作者单位：Zhejiang University; Zhejiang University; Zhejiang University; University of New Brunswick; Westlake University; Westlake University; Brock University; Beijing Life Science Academy; Zhejiang University; Zhejiang University
  摘要：Biosynthesis of similar to 3000 monoterpenoid indole alkaloids (MIAs), including the anticancer drug vinblastine, involves the highly unstable intermediate strictosidine aglycone. Its formation by strictosidine beta-glucosidase (SGD) and subsequent conversion by geissoschizine synthase (GS) occur in spatially separated compartments, representing a major biosynthesis bottleneck. In this study, we discover VinBLAST, a cinnamyl alcohol dehydrogenase-like protein repurposed as a scaffold for effic...
• Ultralong sheathed single-metal-atom chains synthesized under high pressure AI翻译
  作者：Zhang, Jie; Dong, Xiao; Qiu, Shengchao; Yang, Xin; Li, Chengyu; Ma, Hongfei; Fei, Yunfan; Zeng, Qingchao; Li, Fang; Xie, Yi; Duan, Yan; Jiang, Xudong; Xu, Jingqin; Lang, Puyi; Yuan, Jiarui; Luo, Hao; Fang, Yuan; Zhao, Zilin; Bao, Yikun; Wang, Yajie; Chen, Yongjin; Sun, Junliang; Jiang, Shangda; Mao, Ho-kwang; Zheng, Haiyan; Li, Kuo
  作者单位：Nankai University; Peking University; Southern Medical University - China; Quantum Science Center of Guangdong-HongKong-Macao Greater Bay Area
  摘要：Single-metal-atom chains (SMACs) represent the ultimate limit of one-dimensional nanostructures. They serve as archetypal model systems for condensed matter physics and constitute fundamental building blocks for next-generation nanoelectronics. However, synthesis of SMACs suitable for practical applications remains challenging. In this work, we create micrometer-long, carbon-sheathed copper SMACs at milligram scale by compressing beta-copper phthalocyanine to above 21 gigapascals. The SMACs ar...
• China mission aims to probe the Moon's hidden water AI翻译
  作者：Normile, Dennis
• De novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging AI翻译
  作者：Tran, Long; Klein, Steffen; Juergens, David; Sharma, Shajesh; Decarreau, Justin; Lee, Gyu Rie; Wang, Yujia; Chen, Wei; Bera, Asim K.; Kang, Alex; Woods, Jon; Joyce, Emily; Vafeados, Dionne K.; Roullier, Nicole; Li, Xinting; Liu, Bingxu; Bo, Yang; Muratspahic, Edin; Brown, Tim A.; Grimm, Jonathan B.; Patel, Ronak; Lavis, Luke D.; Mahamid, Julia; An, Linna; Baker, David
  作者单位：University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; European Molecular Biology Laboratory (EMBL); University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; Stanford University; University of Washington; University of Washington Seattle; Korea Advanced Institute of Science & Technology (KAIST); Howard Hughes Medical Institute; European Molecular Biology Laboratory (EMBL); Howard Hughes Medical Institute; University of Washington; University of Washington Seattle
  摘要：Fluorescent proteins and small-molecule dyes offer complementary advantages for biological imaging: Proteins are amenable to genetic tagging, whereas dyes provide superior brightness and photostability. To combine these strengths, we used de novo protein design to generate small, nanomolar-affinity, high-selectivity binders (NovoTags) for three cell-permeable dyes spanning the visible spectrum. We show that the NovoTag fluorescent lifetimes can be tuned and demonstrate their application in lif...
• Coding Kids: Big Tech's Battle to Remake Public Schools AI翻译
  作者：Wai, Jonathan
  作者单位：University of Arkansas System; University of Arkansas Fayetteville; University of Arkansas System; University of Arkansas Fayetteville
• RAD51 stabilizes neutrophil extracellular traps to compartmentalize inflammation AI翻译
  作者：Tsansizi, Lorenza Iolanda; Guan, Sophie Yihan; Aramburu, Iker Valle; Punatar, Rajvee Shah; Williams, Thomas J.; Qiao, Yihe E.; Reed, Anna; Armstrong-James, Darius; West, Stephen C.; Papayannopoulos, Venizelos
  作者单位：Francis Crick Institute; Francis Crick Institute; Imperial College London; Guy's & St Thomas' NHS Foundation Trust; Imperial College London; Royal Brompton Hospital; Guy's & St Thomas' NHS Foundation Trust; Royal Brompton & Harefield NHS Foundation Trust; Harefield Hospital
  摘要：Neutrophil extracellular traps (NETs) feature a branched chromatin architecture whose origin and function remain unknown. We found that NET branching is mediated by RAD51, a protein generating DNA junctions during DNA recombination repair. Pharmacological inhibition, RAD51 knockdown, or GEN1 and RuvC resolvase treatment reduced branching and destabilized NETs, whereas RAD51 up-regulation by different stimuli generated NETs with variable stability. RAD51 inhibition during murine pulmonary Asper...
• A native sulfur deposit in Gale crater, Mars AI翻译
  作者：VanBommel, Scott J.; Berger, Jeff A.; King, Penelope L.; Dietrich, William E.; Gellert, Ralf; Thompson, Lucy M.; Vasavada, Ashwin R.; Bryk, Alexander B.; Kite, Edwin S.; Clark, Joanna V.; Cowart, Aster C.; Williams, Rebecca M. E.; Simpson, Sarah L.; Franz, Heather B.; O'Connell-Cooper, Catherine D.; McCraig, Michael A.; Fraeman, Abigail A.; Christian, John R.; Knight, Abigail L.; Boyd, Nicholas I.; Fey, Deirdra M.; Clark, Benton C.; House, Christopher H.
  作者单位：Washington University (WUSTL); National Aeronautics & Space Administration (NASA); NASA Johnson Space Center; Australian National University; University of California System; University of California Berkeley; University of Guelph; University of New Brunswick; National Aeronautics & Space Administration (NASA); California Institute of Technology; NASA Jet Propulsion Laboratory (JPL); University of Chicago; Texas State University System; National Aeronautics & Space Administration (NASA); Texas State University San Marcos; NASA Johnson Space Center; National Aeronautics & Space Administration (NASA); NASA Goddard Space Flight Center; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park
  摘要：Martian rocks are known to contain sulfur-bearing species, including sulfates and sulfides. These compounds record a sulfur cycle that operated over the geological evolution of Mars. We used the Curiosity rover to investigate a deposit of light-toned stones in Gediz Vallis within Gale crater on Mars and found that the stones are composed of native sulfur. The sulfur deposit appears to have formed in place, within a sinuous entrenched canyon cut into the floor of Gediz Vallis. The presence of n...
• Dryland restoration needs shared evidence AI翻译
  作者：Yang, Hong; Gao, Xiang; Wu, Jianhua; Chen, Yao; Yu, Wubin
  作者单位：Zhejiang University of Technology; University of Reading; Zhejiang University of Technology; Zhejiang University; Zhejiang University of Technology; Dalian University of Technology; Zhejiang University of Technology; Zhejiang University of Technology; Zhejiang University of Technology
• Biodiversity monitoring misses behavior AI翻译
  作者：Mikula, Peter; Blumstein, Daniel T.; Tryjanowski, Piotr
  作者单位：Czech University of Life Sciences Prague; University of California System; University of California Los Angeles; Poznan University of Life Sciences
• A Brussels Effect for battery sustainability AI翻译
  作者：Liang, Yanan; Hertwich, Edgar; Istrate, Robert; Valente, Antonio; Wang, Ranran
  作者单位：Leiden University - Excl LUMC; Leiden University; International Institute for Applied Systems Analysis (IIASA); Norwegian University of Science & Technology (NTNU); Nanjing University; Nanjing University