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作者：Perry, Nicholas T.; Bartie, Liam J.; Katrekar, Dhruva; Gonzalez, Gabriel A.; Durrant, Matthew G.; Pai, James J.; Fanton, Alison; Martins, Juliana Q.; Hiraizumi, Masahiro; Ricci-Tam, Chiara; Nishimasu, Hiroshi; Konermann, Silvana; Hsu, Patrick D.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of Tokyo; University of Tokyo; Stanford Medicine; Stanford University; University of California System; University of California Berkeley
摘要：Bridge recombinases are naturally occurring RNA-guided DNA recombinases that we previously demonstrated can programmably insert, excise, and invert DNA in vitro and in Escherichia coli. In this study, we report the discovery and engineering of the bridge recombinase ortholog ISCro4 for universal rearrangements of the human genome. We defined strategies for the optimal application of bridge systems, leveraging mechanistic insights to improve their targeting specificity. Through rational enginee...
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作者：Shen, Chengbo; Ji, Zhe; Jiao, Wu; Zhang, Siyu; Huang, Yunzhi; Qin, Yaojun; Huang, Menghan; Kang, Shuming; Mo, Zulong; Jiang, Bingyu; Yu, Ying; Song, Yajing; Li, Yue; Xu, Jiayi; Tian, Yanan; Xie, Yanjie; Xiong, Guosheng; Wang, Shaokui; Xu, Guohua; Fu, Xiangdong; Li, Shan
作者单位：Nanjing Agricultural University; University of Oxford; Nanjing Forestry University; Nanjing Agricultural University; South China Agricultural University; Chinese Academy of Sciences; Nanjing Institute of Geology & Paleontology, CAS; Institute of Genetics & Developmental Biology, CAS; Nanjing Agricultural University
摘要：Nitrogen (N) deficiency-induced increases in the root-to-shoot biomass ratio in plants are adaptive in nature but suboptimal for agriculture. Understanding the regulatory mechanisms governing this developmental plasticity could help improve crop performance while reducing fertilizer application. We identified OsWRI1a (WRINKLED1a) as a regulatory hub coordinating rice root and shoot growth in response to external N supply, thereby stabilizing the root-to-shoot ratio. In roots, OsWRI1a enhances ...
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作者：Yoshino, Takashi; Sasada, Hiromichi; Sato, Takuya; Nakamura, Tomonori; Shirane, Kenjiro; Ohta, Hiroshi; Kamoshita, Maki; Inoue, Miki; Matsudaira, Yuki; Liu, Chao; Matsufuji, Minatsu; Tachibana, Makoto; Morohashi, Ken-Ichirou; Ikawa, Masahito; Saitou, Mitinori; Ogawa, Takehiko; Hayashi, Katsuhiko
作者单位：University of Osaka; Kyoto University; Kyoto University; Yokohama City University; Kyoto University; University of Osaka; University of Osaka; Azabu University; Kyushu University; University of Osaka; University of Tokyo; Kyoto University
摘要：Proper differentiation of gonadal somatic cells is crucial for sex determination and the production of sex hormones and gametes, and reconstituting this process in culture would both deepen our understanding of this process and enable the generation of gametes in vitro. Here, we report the reconstitution of testicular somatic cells using mouse pluripotent stem cells. The reconstitution recapitulated the sex-determination process, yielding cell types that formed seminiferous tubules and adjacen...
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作者：Beaglehole, Daniel; Radhakrishnan, Adityanarayanan; Boix-Adsera, Enric; Belkin, Mikhail
作者单位：University of California System; University of California San Diego; Massachusetts Institute of Technology (MIT); Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; University of Pennsylvania; University of California System; University of California San Diego
摘要：Artificial intelligence (AI) models contain much of human knowledge. Understanding the representation of this knowledge will lead to improvements in model capabilities and safeguards. Building on advances in feature learning, we developed an approach for extracting linear representations of semantic notions or concepts in AI models. We showed how these representations enabled model steering, through which we exposed vulnerabilities and improved model capabilities. We demonstrated that concept ...
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作者：Li, Qiang; Liu, Ren; Lin, Zuwan; Zhang, Xinhe; Wang, Wenbo; Galicia-Silva, Israeli M.; Liu, Mai; Gao, Zihan; Pollock, Samuel D.; Alvarez-Dominguez, Juan R.; Liu, Jia
作者单位：Harvard University; Harvard University; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Howard Hughes Medical Institute; Pennsylvania Medicine
摘要：Understanding how human pancreatic alpha and beta cell electrical activities mature is critical for building fully functional stem cell-derived (SC-) pancreatic organoids for research and therapeutics. We implanted tissue-like, stretchable electronics during organogenesis of human pancreatic organoids, enabling months-long, single cell-resolved electrophysiology. Longitudinal single-cell tracking suggested that improved hormone responsiveness reflects increasing activity of SC-alpha and -beta ...
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作者：Grimm, David
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作者：Dolgin, Elie
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作者：Opel, Nils; Breakspear, Michael
作者单位：Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin; University of Newcastle; University of Newcastle
摘要：Artificial intelligence (AI) holds transformative potential for the care of people with mental health illnesses. This Review explores key domains and emerging applications of AI in mental health, emphasizing the challenges that must be addressed to ensure safe, effective, and sustainable clinical integration. Distinctive features of mental health care-such as the lack of objective biomarkers, the reliance on behavioral and emotional assessments, the legacy of stigma, and the importance of priv...
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作者：Stahl, Ronit Y.
作者单位：University of California System; University of California Berkeley
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作者：Vaz, Julia
