2024

最新文章

• Targeted demethylation and activation of NLRC5 augment cancer immunogenicity through MHC class I AI翻译
  作者：Sun, Xin; Watanabe, Toshiyuki; Oda, Yoshitaka; Shen, Weidong; Ahmad, Alaa; Ouda, Ryota; de Figueiredo, Paul; Kitamura, Hidemitsu; Tanaka, Shinya; Kobayashi, Koichi S.
  作者单位：Hokkaido University; Hokkaido University; Hokkaido University; Texas A&M University System; Texas A&M University College Station; Texas A&M Health Science Center; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; University of Missouri System; University of Missouri Columbia; Toyo University; Hokkaido University; Hokkaido University
  摘要：Impaired expression of MHC (major histocompatibility complex) class I in cancers constitutes a major mechanism of immune evasion. It has been well documented that the low level of MHC class I is associated with poor prognosis and resistance to checkpoint blockade therapies. However, there is lmited approaches to specifically induce MHC class I to date. Here, we show an approach for robust and specific induction of MHC class I by targeting an MHC class I transactivator (CITA)/NLRC5, using a CRI...
• A novel cysteine-rich adaptor protein is required for mucin packaging and secretory granule stability in vivo AI翻译
  作者：Zhang, Liping; Muirhead, Kayla J.; Syed, Zulfeqhar A.; Dimitriadis, Emilios K.; Hagen, Kelly G. Ten
  作者单位：National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR); National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI); National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB)
  摘要：Mucins are large, highly glycosylated extracellular matrix proteins that line and protect epithelia of the respiratory, digestive, and urogenital tracts. Previous work has shown that mucins form large, interconnected polymeric networks that mediate their biological functions once secreted. However, how these large matrix molecules are compacted and packaged into much smaller secretory granules within cells prior to secretion is largely unknown. Here, we demonstrate that a small cysteine-rich a...
• Single- cell RNA sequencing unveils unique transcriptomic signatures of endothelial cells and role of ENO1 in response to disturbed flow AI翻译
  作者：Chen, Li- Jing; Li, Julie Yi- Shuan; Nguyen, Phu; He, Ming; Chen, Zhen Bouman; Subramaniam, Shankar; Shyy, John Y. - J.; Chien, Shu
  作者单位：University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego; City of Hope; Beckman Research Institute of City of Hope; University of California System; University of California San Diego
  摘要：Flow patterns exert significant effects on vascular endothelial cells (ECs) to lead to the focal nature of atherosclerosis. Using a step flow chamber to investigate the effects of disturbed shear (DS) and pulsatile shear (PS) on ECs in the same flow channel, we conducted single - cell RNA sequencing analyses to explore the distinct transcriptomic profiles regulated by DS vs. PS. Integrated analysis identified eight cell clusters and demonstrated that DS induces EC transition from atheroprotect...
• miR-137 regulates PTP61F, affecting insulin signaling, metabolic homeostasis, and starvation resistance in Drosophila AI翻译
  作者：Saedi, Hana; Waro, Girma; Giacchetta, Lea; Tsunoda, Susan
  作者单位：Colorado State University System; Colorado State University Fort Collins
  摘要：miR-137is a highly conserved brain- enriched microRNA (miRNA) that has been associated with neuronal function and proliferation. Here, we show that Drosophila miR-137 null mutants display increased body weight with enhanced triglyceride content and decreased locomotor activity. In addition, when challenged by nutrient deprivation, miR-137 mutants exhibit reduced motivation to feed and prolonged survival. We show through genetic epistasis and rescue experiments that this starvation resistance i...
• Siglec-15/sialic acid axis as a central glyco- immune checkpoint in breast cancer bone metastasis AI翻译
  作者：Wang, Yixian; Xu, Zhan; Yu, Liqun; Wang, Chenhang; Ding, Haoxue; Gao, Yang; Sun, Han; Xia, Meng; Chen, Yuda; Wu, Kuan- Lin; Wu, Yi-Hsuan; Xiao, Han
  作者单位：Rice University; Baylor College of Medicine; Rice University; Rice University
  摘要：Immunotherapy is a promising approach for treating metastatic breast cancer (MBC), offering new possibilities for therapy. While checkpoint inhibitors have shown great progress in the treatment of metastatic breast cancer, their effectiveness in patients with bone metastases has been disappointing. This lack of efficacy seems to be specific to the bone environment, which exhibits immunosuppressive features. In this study, we elucidate the multiple roles of the sialic acid-binding Ig-like lecti...
• Synonymous edits in the Escherichia coli genome have substantial and condition- dependent effects on fitness AI翻译
  作者：Yang, Dong-Dong; Rusch, Leo M.; Widney, Karl A.; Morgenthaler, Andrew B.; Copley, Shelley D.
  作者单位：University of Colorado System; University of Colorado Boulder; University of Colorado System; University of Colorado Boulder
  摘要：CRISPR-Cas-based genome editing is widely used in bacteria at scales ranging from construction of individual mutants to massively parallel libraries. This procedure relies on guide RNA- directed cleavage of the genome followed by repair with a template that introduces a desired mutation along with synonymous immunizing mutations to prevent re- cleavage of the genome after editing. Because the immunizing mutations do not change the protein sequence, they are often assumed to be neutral. However...
• The space-for-time gambit fails a robust test AI翻译
  作者：Angert, Amy L.
  作者单位：University of British Columbia
• SAGA1 and SAGA2 promote starch formation around proto-pyrenoids in Arabidopsis chloroplasts AI翻译
  作者：Atkinson, Nicky; Stringer, Rhea; Mitchell, Stephen R.; Seung, David; McCormick, Alistair J.
  作者单位：University of Edinburgh; University of Edinburgh; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); John Innes Center
  摘要：The pyrenoid is a chloroplastic microcompartment in which most algae and some terrestrial plants condense the primary carboxylase, Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) as part of a CO2-concentrating mechanism that improves the efficiency of CO2 capture. Engineering a pyrenoid-based CO2-concentrating mechanism (pCCM) into C3 crop plants is a promising strategy to enhance yield capacities and resilience to the changing climate. Many pyrenoids are characterized by a sheath of...
• Climate change determines the sign of productivity trends in US forests AI翻译
  作者：Hogan, Aaron; Domke, Grant M.; Zhu, Kai; Johnson, Daniel J.; Lichstein, Jeremy W.
  作者单位：State University System of Florida; University of Florida; United States Department of Agriculture (USDA); United States Forest Service; University of Michigan System; University of Michigan; State University System of Florida; University of Florida
  摘要：Forests are integral to the global land carbon sink, which has sequestered -30% of anthropogenic carbon emissions over recent decades. The persistence of this sink depends on the balance of positive drivers that increase ecosystem carbon storage-e.g., CO2 fertilization-and negative drivers that decrease it-e.g., intensifying disturbances. The net response of forest productivity to these drivers is uncertain due to the challenge of separating their effects from background disturbance-regrowth d...
• Building on-chip cytoskeletal circuits via branched microtubule networks AI翻译
  作者：Zaferani, Meisam; Song, Ryungeun; Petry, Sabine; Stone, Howard A.
  作者单位：Princeton University; Princeton University; Princeton University
  摘要：Controllable platforms to engineer robust cytoskeletal scaffolds have the potential to create novel on-chip nanotechnologies. Inspired by axons, we combined the branching microtubule (MT) nucleation pathway with microfabrication to develop cytoskeletal circuits. This active matter platform allows control over the adaptive self-organization of uniformly polarized MT arrays via geometric features of microstructures designed within a microfluidic confinement. We build and characterize basic eleme...