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作者：Horton, Connor A.; Alexandari, Amr M.; Hayes, Michael G. B.; Marklund, Emil; Schaepe, Julia M.; Aditham, Arjun K.; Shah, Nilay; Suzuki, Peter H.; Shrikumar, Avanti; Afek, Ariel; Greenleaf, William J.; Gordan, Raluca; Zeitlinger, Julia; Kundaje, Anshul; Fordyce, Polly M.
作者单位：Stanford University; Stanford University; Stanford University; Stanford University; Stowers Institute for Medical Research; Duke University; Duke University; Weizmann Institute of Science; Duke University; Duke University; University of Kansas; University of Kansas Medical Center; Chan Zuckerberg Initiative (CZI); University of California System; University of California Berkeley; Fred Hutchinson Cancer Center
摘要：Short tandem repeats (STRs) are enriched in eukaryotic cis-regulatory elements and alter gene expression, yet how they regulate transcription remains unknown. We found that STRs modulate transcription factor (TF)-DNA affinities and apparent on-rates by about 70-fold by directly binding TF DNA-binding domains, with energetic impacts exceeding many consensus motif mutations. STRs maximize the number of weakly preferred microstates near target sites, thereby increasing TF density, with impacts we...
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作者：Lim, Yang; Tamayo-Orrego, Lukas; Schmid, Ernst; Tarnauskaite, Zygimante; Kochenova, Olga V.; Gruar, Rhian; Muramatsu, Sachiko; Lynch, Luke; Schlie, Aitana Verdu; Carroll, Paula L.; Chistol, Gheorghe; Reijns, Martin A. M.; Kanemaki, Masato T.; Jackson, Andrew P.; Walter, Johannes C.
作者单位：Harvard University; Harvard Medical School; University of Edinburgh; Howard Hughes Medical Institute; Research Organization of Information & Systems (ROIS); National Institute of Genetics (NIG) - Japan; Stanford University; Stanford University; Graduate University for Advanced Studies - Japan; University of Tokyo; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); Babraham Institute
摘要：CDC45-MCM2-7-GINS (CMG) helicase assembly is the central event in eukaryotic replication initiation. In yeast, a multi-subunit pre-loading complex (pre-LC) accompanies GINS to chromatin-bound MCM2-7, leading to CMG formation. Here, we report that DONSON, a metazoan protein mutated in microcephalic primordial dwarfism, is required for CMG assembly in vertebrates. Using AlphaFold to screen for protein-protein interactions followed by experimental validation, we show that DONSON scaffolds a verte...
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作者：Pena-Claros, Marielos; Nobre, Carlos
作者单位：Wageningen University & Research; Universidade de Sao Paulo
摘要：Early in August this year, a high-profile summit was held in Belem, Brazil, where the eight Amazonian countries discussed the future of the Amazon. The nations recognized that the Amazon is very close to reaching a tipping point for turning into a degraded ecosystem. The result of their discussions was the Belem Declaration, an ambitious plan to protect and conserve the Amazon forests and to support Indigenous Peoples and local communities. Concern arose, however, because they failed to agree ...
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作者：Trapani, Josh
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作者：Trumbo, Samantha K.; Brown, Michael E.
作者单位：Cornell University; California Institute of Technology
摘要：Jupiter's moon Europa has a subsurface ocean, the chemistry of which is largely unknown. Carbon dioxide (CO2) has previously been detected on the surface of Europa, but it was not possible to determine whether it originated from subsurface ocean chemistry, was delivered by impacts, or was produced on the surface by radiation processing of impact-delivered material. We mapped the distribution of CO2 on Europa using observations obtained with the James Webb Space Telescope (JWST). We found a con...
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作者：Dickman, Jamie
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作者：Gemen, Julius; Church, Jonathan R.; Ruoko, Tero-Petri; Durandin, Nikita; Bialek, Michal J.; Weienfels, Maren; Feller, Moran; Kazes, Miri; Odaybat, Magdalena; Borin, Veniamin A.; Kalepu, Rishir; Diskin-Posner, Yael; Oron, Dan; Fuchter, Matthew J.; Priimagi, Arri; Schapiro, Igor; Klajn, Rafal
作者单位：Weizmann Institute of Science; Hebrew University of Jerusalem; Tampere University; Imperial College London; Weizmann Institute of Science; Institute of Science & Technology - Austria
摘要：Photoisomerization of azobenzenes from their stable E isomer to the metastable Z state is the basis of numerous applications of these molecules. However, this reaction typically requires ultraviolet light, which limits applicability. In this study, we introduce disequilibration by sensitization under confinement (DESC), a supramolecular approach to induce the E-to-Z isomerization by using light of a desired color, including red. DESC relies on a combination of a macrocyclic host and a photosen...
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作者：Kalnaus, Sergiy; Dudney, Nancy J.; Westover, Andrew S.; Herbert, Erik; Hackney, Steve
作者单位：United States Department of Energy (DOE); Oak Ridge National Laboratory; United States Department of Energy (DOE); Oak Ridge National Laboratory; United States Department of Energy (DOE); Oak Ridge National Laboratory; Michigan Technological University
摘要：Solid-state batteries with lithium metal anodes have the potential for higher energy density, longer lifetime, wider operating temperature, and increased safety. Although the bulk of the research has focused on improving transport kinetics and electrochemical stability of the materials and interfaces, there are also critical challenges that require investigation of the mechanics of materials. In batteries with solid-solid interfaces, mechanical contacts, and the development of stresses during ...
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作者：Brainard, Jeffrey
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作者：Couzin-Frankel, Jennifer
摘要：Svetlana Mojsov helped discover the hormone GLP-1, paving the way for blockbuster obesity drugs. Now, she's fighting for recognition
