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作者:Levy, Joshua I.; Andersen, Kristian G.; Knight, Rob; Karthikeyan, Smruthi
作者单位:Scripps Research Institute; University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego
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作者:Normile, Dennis
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作者:Ren, Zhongshu; Gao, Lin; Clark, Samuel J.; Fezzaa, Kamel; Shevchenko, Pavel; Choi, Ann; Everhart, Wes; Rollett, Anthony D.; Chen, Lianyi; Sun, Tao
作者单位:University of Virginia; United States Department of Energy (DOE); Argonne National Laboratory; United States Department of Energy (DOE); Honeywell; Carnegie Mellon University; University of Wisconsin System; University of Wisconsin Madison
摘要:Porosity defects are currently a major factor that hinders the widespread adoption of laser-based metal additive manufacturing technologies. One common porosity occurs when an unstable vapor depression zone (keyhole) forms because of excess laser energy input. With simultaneous high-speed synchrotron x-ray imaging and thermal imaging, coupled with multiphysics simulations, we discovered two types of keyhole oscillation in laser powder bed fusion of Ti-6Al-4V. Amplifying this understanding with...
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作者:Singh, Garima
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作者:Tan, Bryce W. Q.
作者单位:National University of Singapore
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作者:Tan, Bryce W. Q.
作者单位:National University of Singapore
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作者:Brainard, Jeffrey
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作者:Mangum, Kevin D.; Gallagher, Katherine A.
作者单位:University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
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作者:Brainard, Jeffrey
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作者:Djenoune, Lydia; Mahamdeh, Mohammed; V. Truong, Thai; Nguyen, Christopher T.; Fraser, Scott E.; Brueckner, Martina; Howard, Jonathon; Yuan, Shiaulou
作者单位:Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard Medical School; Cleveland Clinic Foundation; Massachusetts Institute of Technology (MIT); Yale University; Yale University
摘要:The breaking of bilateral symmetry in most vertebrates is critically dependent upon the motile cilia of the embryonic left-right organizer (LRO), which generate a directional fluid flow; however, it remains unclear how this flow is sensed. Here, we demonstrated that immotile LRO cilia are mechanosensors for shear force using a methodological pipeline that combines optical tweezers, light sheet microscopy, and deep learning to permit in vivo analyses in zebrafish. Mechanical manipulation of imm...