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作者：Kolyadko, Vladimir N.; Pumroy, Ruth A.; Moiseenkova-Bell, Vera Y.; Krishnaswamy, Sriram
作者单位：University of Pennsylvania; Pennsylvania Medicine; Childrens Hospital of Philadelphia; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine
摘要：Phosphatidylserine on the surface of activated cells serves as a signal for coagulation factor binding and the membrane-dependent assembly of enzyme complexes that drive the accelerated host response to vascular damage. Here, we use single-particle cryo-EM of liposome-bound proteins to establish common mechanisms utilized by coagulation factors V and VIII for membrane binding and phospholipid specificity. The interface between these peripheral proteins and the membrane shows contacts between t...
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作者：Zhang, Zhengyang; Zhang, Liwei; An, Peng; Zhang, Xu; Xia, Yi; Kang, Yunlu; Chen, Xiaoxia; Hua, Rongrong; Zhu, Yinhua; Hao, Yanling; Huang, Yuan; Luo, Yongting; Luo, Junjie; Wang, Guisheng
作者单位：China Agricultural University; Peking University; Chinese People's Liberation Army General Hospital; Chinese Academy of Medical Sciences - Peking Union Medical College; Fu Wai Hospital - CAMS; Chinese Academy of Medical Sciences - Peking Union Medical College; Fu Wai Hospital - CAMS; Peking Union Medical College
摘要：Simultaneously profiling mitochondrial DNA (mtDNA) heteroplasmy and phenotypic variability at the single-cell level remains a challenge due to the absence of integrated methods that map mitochondrial genotypes alongside their functional states. We introduce human single-cell mitochondrial phenotype-coupled mtDNA sequencing (scMPCDS), a platform that quantifies mtDNA mutations and heteroplasmy together with mitochondrial membrane potential and reactive oxygen species within individual cells. Un...
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作者：Xu, Guanghui; Fu, Yanmei; Lin, Xiaolong; Huang, Xinrong; Yu, Yong; de Vries, Franciska T.; Rillig, Matthias C.
作者单位：Chinese Academy of Sciences; Northeast Institute of Geography & Agroecology, CAS; Free University of Berlin; Sun Yat Sen University; Chinese Academy of Sciences; Institute of Urban Environment, CAS; University of Amsterdam
摘要：Antimicrobial resistance (AMR) is a growing One Health challenge, and as climate warming intensifies extreme events, it remains unclear how these disturbances affect soil antibiotic resistance genes (ARGs). Here we analyzed the data from a controlled experiment using soils from 30 grassland sites across ten European countries, which simulated drought, flooding, freeze-thaw, and heatwaves to explore ARG dynamics. Overall, ARGs exhibited relatively small but highly consistent shifts across treat...
