Single-shot wide-field biochemical imaging at 1 kHz frame rate

• 成果类型: Article
• 署名作者: Wang, Jizhou; Marshall, Nathan; Han, Zehua; Wang, Kai; Sprague, Richard; Yi, Zhenhuan; Yu, Wenxuan; Xu, Xingqi; He, Zhe; Wang, Da-Wei; Scully, Marlan O.; Sokolov, Alexei V.
• 署名单位: Texas A&M University System; Texas A&M University College Station; Texas A&M University System; Texas A&M University College Station; Zhejiang University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2603591123
• 发表日期: 2026-07-07
• 页码: e2603591123
• 关键词: wide-field; mid-infrared; spectroscopy; biochemical imaging; single-shot; c-elegans; raman; microscopy; MODEL

摘要

Vibrational microspectroscopy, including both Raman-based and infrared-based techniques, can map the chemical distribution of samples based on molecular vibrations without labeling. However, imaging fast dynamics in living organisms remains challenging. To address this, we propose a wide-field infrared microspectroscopy capable of single-shot imaging, where each image is captured with a single pair of laser pulses lasting approximately one picosecond. It minimizes motion blur and allows observing fast dynamic processes at frame rates up to the laser repetition rate. This approach is based on the infrared-resonant third-order sum-frequency process, which converts infrared light to visible signals. We demonstrate the capability through single-shot in vivo imaging of alive Caenorhabditis elegans worms in water, achieving a spatial resolution of approximately 400 nm. Additionally, 1,000 Hz single-shot videos of moving worms are shown by using a kHz laser system. This approach opens more possibilities for imaging chemicals involved in fast dynamic processes, offering diverse applications in both chemistry and biology.