PLETHORA-autophagy axis activates organ regeneration through ROS modulation

• 成果类型: Article
• 署名作者: Ganguly, Akansha; Humnabadkar, Aabha; Gautam, Komal; Willemsen, Viola; Xu, Lin; Dagdas, Yasin; Prasad, Kalika
• 署名单位: Indian Institute of Science Education & Research (IISER) Pune; Wageningen University & Research; Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS; Ruprecht Karls University Heidelberg
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2513954123
• 发表日期: 2026-02-10
• 页码: e2513954123
• 关键词: novo root regeneration; autophagy; PLETHORA; ros; stem cell; NOVO ROOT ORGANOGENESIS; cell fate transition; PLANT AUTOPHAGY; arabidopsis; chloroplasts; proteins; vacuole; signals; ATG8; mechanisms

摘要

Injury-induced disruption of cellular homeostasis leads to accumulation of stress at sites adjacent to a wound. How do these cells mitigate wound-induced stress and restore cellular homeostasis to promote regeneration? To address this question, we examined the role of autophagy-a conserved quality control pathway that recycles defective cellular components to sustain cellular homeostasis-in facilitating wound repair in plants. We demonstrate that transcriptional activation of autophagy-related gene 8 (ATG8) genes is essential for de novo root regeneration, but dispensable for wound induced callus formation from an excised leaf. Plant-specific transcription factors PLETHORA (PLT) activate the transcription of a subset of ATG8 genes and function nonredundantly turnover, resulting in increased intracellular stress and ectopic accumulation of reactive motes the expression of stem-cell regulators for successful de novo root regeneration. aging wound-induced cellular stress and facilitating organ regeneration.