Cellular responses to FGF1 are modulated by palmitoylation of the docking protein FRS2α

• 成果类型: Article
• 署名作者: An, Seong J.; Suzuki, Yoshihisa; Mohanty, Jyotidarsini; Tome, Francisco; Lax, Irit; Schlessinger, Joseph
• 署名单位: Yale University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2605311123
• 发表日期: 2026-05-05
• 页码: e2605311123
• 关键词: receptor tyrosine kinases; cell signaling; palmitoylation; myristoylation; phosphorylation; FIBROBLAST-GROWTH-FACTOR; factor receptor; map kinase; FRS2; binding; macropinocytosis; activation; expression; CORTACTIN; patterns

摘要

An important mechanism by which receptor tyrosine kinases (RTKs) mediate cellular responses involves the formation of signaling complexes through direct interactions with membrane-associated docking proteins, followed by phosphorylation of multiple tyrosine residues. These docking proteins recruit and activate downstream signaling molecules and enzymes following ligand stimulation. The docking protein FRS2 alpha has been established as a major signaling hub activated by fibroblast growth factors (FGFs), neurotrophic factors, and other extracellular cues. Here, we show that palmitoylation of FRS2 alpha at two sites is essential for stabilizing its myristoylation-dependent association with the plasma membrane. FGF1-induced mitogen-activated protein kinase (MAPK) activation and other cellular responses are partially restored in cells expressing FRS2 alpha mutants deficient in either one of the two palmitoylation sites. However full restoration of signal strength including MAPK response and other FGF1-induced cellular activities requires palmitoylation at both FRS2 alpha sites. In addition to enhancing signaling robustness, anchoring of FRS2 alpha to the plasma membrane creates a structural platform for assembling multiprotein complexes essential for cytoskeletal reorganization associated with membrane ruffling, macropinocytosis, and other FGF1-induced processes. Finally, we demonstrate that while PC12 cells lacking FRS2 alpha or deficient in FRS2 alpha palmitoylation can proliferate, FGF1-induced neuronal differentiation strictly depends on the palmitoylation of the docking protein.