Biomolecular condensation of ERC1 recruits ATG8 and NBR1 to drive autophagosome formation for plant heat tolerance

• 成果类型: Article
• 署名作者: Chung, Ka Kit; Law, Kai Ching; Zhao, Ziwei; Ma, Juncai; Zhan, Xiao-Tong; Chiang, Cheuk Him; Leung, Kwan Ho; Shrestha, Ruben; Wu, Yixin; Li, Chaorui; Lee, Ka Ming; Feng, Lei; Li, Xibao; Wong, Kam Bo; Xu, Shou-Ling; Gao, Caiji; Zhuang, Xiaohong
• 署名单位: Chinese University of Hong Kong; Chinese University of Hong Kong; Carnegie Institution for Science; Chinese University of Hong Kong; South China Normal University; South China Normal University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2425689122
• 发表日期: 2025-11-18
• 页码: e2425689122
• 关键词: ERC protein family; biomolecular condensation; ATG8; autophagosome formation; NBR1; phosphatidylinositol 3-phosphate; phase-separation; active zone; proteins

摘要

Macroautophagy (hereafter autophagy) is essential for cells to respond to nutrient deficiency by delivering cytosolic contents to vacuoles for degradation via the formation of a multilayer organelle named an autophagosome. A set of autophagy-related (ATG) regulators are recruited to the phagophore assembly site for phagophore initiation, including its expansion and closure, and subsequent delivery into the vacuole. However, it remains elusive how the phagophore assembly is regulated under different stress conditions. Here, we described an uncharacterized Arabidopsis (Arabidopsis thaliana) ERC (ELKS/Rabb-interacting/CAST) protein family as an interacting partner ofATG8. ERC1 proteins translocate to the phagophore membrane and develop into ring-like autophagosomes upon autophagic induction. Notably, we found that ERC1 proteins possess the ability to assemble into substantial droplets together with ATG8e proteins prior to ATG8 conjugation to the membrane. Through multiscale characterization, we demonstrated that the ERC1 membraneless droplet represents a distinct type of plant condensate. Additionally, ERC1 directly binds to NBR1 to promote NBR1 degradation. ERC1 dysfunction suppresses the turnover of ubiquitinated substrates and compromises plant tolerance to heat stress. Our study suggests a model for autophagic degradation in response to heat stress by the action of ERC1-mediated biomolecular condensation in Arabidopsis.