Ubiquitination of BAM1 attenuates CLE peptide-mediated signaling in the root apical meristem

• 成果类型: Article
• 署名作者: Zhou, Yuanyuan; Liu, Fei; Han, Yongfeng; Bai, Jiaojiao; Zhang, Baowen; Tang, Wenqiang; Gou, Xiaoping; Zhang, Yan; Lu, Dongping
• 署名单位: Shanghai Jiao Tong University; Chinese Academy of Sciences; Center for Agricultural Resources Research, Institute of Genetic & Developmental Biology, CAS; Institute of Genetics & Developmental Biology, CAS; Nankai University; Hebei Normal University; Lanzhou University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2530116123
• 发表日期: 2026-01-27
• 页码: e2530116123
• 关键词: BAM1; ubiquitination; apical meristem; RGLG1/2; CLE13; arabidopsis; RECOGNITION; GROWTH; perception; flagellin; proteins; requires; RGLG1; FLS2

摘要

The plasma membrane-resident receptor-like kinases (RLKs) and their cognate peptide ligands play crucial roles in plant growth and development. The RLK BARELY ANY MERISTEM1 (BAM1) promotes phloem formation and regulates other aspects of root development. However, the mechanisms governing BAM1 protein degradation remain unclear. In this study, we demonstrate that two closely related ubiquitin ligases, RING DOMAIN LIGASE 1 (RGLG1) and RGLG2, specifically interact with BAM1 and its closest homolog BAM2. RGLG1/2 ubiquitinate BAM1/2 and mediate their degradation, thereby dampening BAM1/2 signaling. Treatment with the peptide CLE13 (CLV3/EMBRYO SURROUNDING REGION-RELATED 13) enhances the BAM1/2-RGLG2 interaction and the ubiquitin ligase activity of RGLG2, resulting in increased ubiquitination and degradation of BAM1/2 by RGLG1/2. The rglg1 rglg2 double mutant exhibits increased sensitivity to CLE13 compared to the wild type. Collectively, our findings demonstrate that RGLG1/2-mediated ubiquitination and degradation of BAM1/2 attenuate CLE13-mediated signaling in root meristem.