From Drosophila to mammals: The evolutionarily conserved homeodomain protein Engrailed affects the antitumor innate immune response

• 成果类型: Article
• 署名作者: Huang, Jieqing; Lan, Xiao; Huang, Xiaoming; Yuan, Yuntong; Liu, Mo; Liu, Jiyong; Chen, Juanjuan; Ye, Zhenhui; Wei, Ziming; Chen, Yuqiang; Deng, Zihao; Zhang, Yuhong; He, Yanzhen; Zhou, Xiaojing; Cao, Zhenming; Jiu, Mengyu; Jiao, Renjie; Cai, Hua; Hoffmann, Jules A.; Chen, Di
• 署名单位: Guangzhou Medical University; Universites de Strasbourg Etablissements Associes; Universite de Strasbourg; Centre National de la Recherche Scientifique (CNRS); Universites de Strasbourg Etablissements Associes; CNRS - National Institute for Biology (INSB); Universite de Strasbourg
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2603878123
• 发表日期: 2026-04-28
• 页码: e2603878123
• 关键词: innate immunity; antitumor defense; Engrailed; homeodomain protein; Drosophila melanogaster; homeotic genes; SEQUENCE HOMOLOGY; dna-sequence; RECOGNITION; melanogaster; expression; ANTENNAPEDIA; COMPARTMENT; bacterial; locus

摘要

In the process of investigating innate immune reactions against injected oncogenic cells in Drosophila, we noted that the homeodomain protein Engrailed, known since last century for its role in segmentation polarity in embryos, is reactivated in adult males where it considerably restricts the expected proliferation of these cells. Importantly, this effect was not induced following injection of selected pathogens or primary Drosophila embryonic cells. We show that the injection of these cells induces the nuclear translocation of the cytosolic Engrailed protein, and we identify a series of downstream effector genes which are either positively or negatively regulated by this transcription factor. Our results point to unexpected functional parallels between engrailed genes in mammals and flies, which is further illustrated by the phenotypes induced in fly wings and tumor load after ectopic expression of mammalian genes in flies.