Species- specific roles of SP1 in bovine and human embryonic genome activation and early embryonic development
成果类型：
Article
署名作者：
Jin, Hao; Xiao, Lieying; He, Xiaolin; Hu, Bingjie; Shi, Yan; Xu, Bo; Tong, Xiaomei; Wang, Shaohua; Zhao, Panpan; Luo, Lei; Zhang, Kun
署名单位：
Zhejiang University; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Zhejiang University; University of California System; University of California Los Angeles; David Geffen School of Medicine at UCLA; University of California Los Angeles Medical Center; Michigan State University; Fudan University
刊物名称：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN/ISSBN：
0027-8424; 1091-6490
DOI：
10.1073/pnas.2526998123
发表日期：
2026-03-24
页码：
e2526998123
关键词：
EGA
human embryo
bovine embryo
preimplantation
SP1
TRANSCRIPTION FACTOR SP1
MITHRAMYCIN
landscape
chromatin
alignment
oocytes
lineage
binding
gene
摘要：
Embryonic genome activation (EGA) marks the onset of the embryonic program and enables the transition toward the first lineage specification. However, the molecular features of EGA and the transcription factors (TFs) orchestrating this process remain unclear. Here, by performing single- cell RNA- seq on bovine embryos, we reveal that major EGA is asynchronously initiated among blastomeres at the 8- cell stage. Integrative analyses reveal distinct protein accumulation compared with transcriptional and translational activation during bovine EGA. Furthermore, we investigate the role of SP1, a TF activated at the minor EGA stage, with motifs enriched in accessible chromatin during the major EGA stage in bovine and human embryos. SP1 deficiency leads to morula arrest in bovine and impairs EGA in human embryos. Multiomics analysis demonstrates in bovine and directly targets key EGA genes in human embryos. Together, our study delineates the dynamics of bovine EGA and uncovers the conserved and species- specific roles of SP1 in regulating EGA and early development in mammals.
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