Lipid droplets confer SA-dependent antiviral defense without growth penalty

• 成果类型: Article
• 署名作者: Zhou, Lifan; Wen, Zhiyan; Zhong, Chenchen; Zhang, Dingliang; Zhang, Qianshen; Wang, Ruiqi; Duan, Jiangning; Zhang, Kun; Li, Zhen; Zhao, Xiaoyun; Mei, Aodong; Wang, Yuanzhi; Zhou, Tong; Du, Linlin; Chu, Jinfang; Cheng, Shujing; Zhao, Xiaofei; Yang, Meng; Li, Dawei; Dinesh-Kumar, Savithramma P.; Zhang, Yongliang
• 署名单位: China Agricultural University; Yangzhou University; Jiangsu Academy of Agricultural Sciences; Chinese Academy of Sciences; Institute of Genetics & Developmental Biology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; University of California System; University of California Davis; University of California System; University of California Davis
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2600696123
• 发表日期: 2026-08-18
• 页码: e2600696123
• 关键词: lipid droplets; LD-associated proteins 2.1; barley stripe mosaic virus; salicylic acid; DEFENSE; TRIACYLGLYCEROL ACCUMULATION; beta-oxidation; acyltransferase; promotes

摘要

The role of lipid droplets (LDs), typically regarded as storage sites for neutral lipid, in plant defense remains poorly understood. In our study with barley stripe mosaic virus (BSMV), a (+)ssRNA virus, we found that infection significantly increases LD abundance in Nicotiana benthamiana and wheat. Using split-TurboID-based proximity labeling, we identified LD-associated proteins (LDAPs) at ER-chloroplast contact sites. Increasing LD formation by overexpressing mouse FIT2 or plant NbLDAP2.1 decreased viral accumulation, whereas impairing LD formation led to higher infection. Live cell imaging and genetic studies revealed that LDs move along actin filaments, cluster around chloroplasts, and contribute to antiviral immunity through a salicylic acid (SA)-dependent mechanism. Moreover, LDs conferred resistance to multiple (+)ssRNA viruses in N. benthamiana and a (-)ssRNA virus in rice. These findings uncover a role for LDs in SA-dependent antiviral defense that does not compromise growth, offering potential approaches for engineering virus-resistant crops.