The inferred functional connectome underlying circadian synchronization in the mouse suprachiasmatic nucleus

• 成果类型: Article
• 署名作者: Nikhil, K. L.; Singhal, Bharat; Granados-Fuentes, Daniel; Li, Jr - Shin; Kiss, Istvan Z.; Herzog, Erik D.
• 署名单位: Washington University (WUSTL); Washington University (WUSTL); Saint Louis University; Washington University (WUSTL)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2520674122
• 发表日期: 2025-12-16
• 页码: e2520674122
• 关键词: connectome; Information theory; circadian; suprachiasmatic nucleus; vasoactive intestinal peptide; VASOACTIVE INTESTINAL POLYPEPTIDE; CELLULAR CLOCKS; gene-expression; neurons; oscillators; rhythms; models; cells; reconstruction; Heterogeneity

摘要

Circadian rhythms in mammals arise from the spatiotemporal synchronization of similar to 20,000 neuronal clocks in the suprachiasmatic nucleus (SCN). Although anatomical, molecular, and genetic approaches have revealed diverse SCN cell types, how network-level wiring enables their synchronization remains unclear. To overcome the challenges of inferring functional connectivity from fixed tissue, we developed Mutual Information & Transfer Entropy (MITE), an information-theoretic framework to infer directed cell-cell connections with high fidelity from long-term live-cell imaging. Recording and analyzing 3,290 h of clock gene expression from 8,261 SCN neurons across 17 mice, we uncovered a highly conserved, sparse SCN network organized into two asymmetrically coupled modules: dorsal and ventral. Connectivity analyses revealed five functional SCN cell types independent of neurochemical identity. Notably, only similar to 30% of vasoactive intestinal peptide neurons exhibited Hub-like connectivity, classifying them as Generators and Broadcasters of synchrony signals. Other spatially stereotyped cell types consistently identified as Bridges, Receivers, or Sinks. Simulations based on MITE-inferred connectomes recapitulated emergent SCN dynamics, including recovery from desynchrony and the daily dorsal-to-ventral phase wave of gene expression. Together, these results demonstrate that MITE enables precise mapping of cellular network topology, revealing the circuit logic and key cell types that mediate circadian synchrony across