Loss of calcium-dependent protein kinases OsCPK5 and OsCPK13 leads to NLR-dependent resistance in rice

• 成果类型: Article
• 署名作者: Wang, Zhanchun; Yu, Shibo; Xu, Wencai; Peng, Han; Zhou, Xuan; Liese, Anja; Chen, Lilan; Zhong, Guitao; Zhong, Chen; Deng, Xianya; Han, Libo; Liu, Na; Lee, Justin; Romeis, Tina; Tang, Dingzhong; Wang, Wei
• 署名单位: Fujian Agriculture & Forestry University; Fujian Agriculture & Forestry University; Fujian Agriculture & Forestry University; Fujian Agriculture & Forestry University; Leibniz Institut fur Pflanzenbiochemie; Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2506856122
• 发表日期: 2025-11-11
• 页码: e2506856122
• 关键词: ca2+; OsCPK5; NLR protein; cell death; rice blast resistance; plant immunity; rin4

摘要

Calcium (Ca2+) signaling plays a crucial role in plant immunity, regulating both pattern-triggered immunity (PTI) through cell-surface receptors and effector-triggered immunity (ETI) via intracellular nucleotide-binding, leucine-rich repeat receptors (NLRs). Calcium-dependent protein kinases (CPKs/CDPKs) serve as key Ca2+ sensors and signal transducers in these processes. In this study, we demonstrate the relevance of two rice CPKs, OsCPK5 and OsCPK13, for rice blast resistance. Both are Ca2+-responsive kinases, with potential in planta heteromer formation enhancing their phosphorylation/ signaling functions. Single oscpk5 and oscpk13 mutants exhibit impaired early PTI responses and enhanced susceptibility to rice blast fungus, suggesting that these kinases are essential for effective immunity. Surprisingly, although it is also defective in PTI, the oscpk5/13 double mutant displays enhanced resistance to rice blast. An NLR protein OsCPK5/13-ASSOCIATING RESISTANCE PROTEIN 1 (OsCARP1), which is physically associated with both OsCPK5 and OsCPK13, is genetically required for the heightened resistance of oscpk5/13. Furthermore, OsCARP1-induced cell death in Nicotiana benthamiana can be suppressed by the expression of OsCPK5 and OsCPK13. Based on these findings, we postulate that the positive blast resistance roles of OsCPK5 and OsCPK13 are guarded by OsCARP1, thus leading to OsCARP1-dependent ETI resistance in the oscpk5/13 double mutant or upon manipulation by still unknown pathogen effectors during infection. Our results offer insights into how plants counteract potential pathogen attack on key Ca2+ signaling immune components.