Recruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate

• 成果类型: Article
• 署名作者: Ko, Donghwi; Ruonala, Raili; Faille, Alexandre; Hellmann, Eva; Help, Hanna; Liu, Huili; Nielsen, Ronni; Haakonsson, Anders; De Diego, Nuria; Paatero, Anja; Shcherbii, Mariia V.; Stefanowicz, Karolina; Cavar Zeljkovic, Sanja; Drud Lundager Rasmussen, Tine; Novak, Ondrej; Bodi, Zsuzsanna; Eswaran, Gugan; Wybouw, Brecht; Bourdon, Matthieu; Urbez, Cristina; Liu, Xiaonan; Salokas, Kari; Ohman, Tiina; Waldie, Tanya; Toronen, Petri; El-Showk, Sedeer; Balcerowicz, Martin; Besnard, Fabrice; Liu, Xiaomin; Perkins, Patrick; Mazzoni-Putman, Serina; Vainonen, Julia P.; Sierla, Maija; Frilander, Mikko J.; Mandrup, Susanne; Vernoux, Teva; Ljung, Karin; Ferrando, Alejandro; Blazquez, Miguel A.; Holm, Liisa; Fray, Rupert; Varjosalo, Markku; Leyser, Ottoline; Paavilainen, Ville O.; Mahonen, Ari Pekka; Stepanova, Anna; Alonso, Jose; Heber, Steffen; Malinowski, Robert; Kirpekar, Finn; Warren, Alan J.; Helariutta, Yka
• 署名单位: University of Helsinki; University of Cambridge; University of Helsinki; University of Cambridge; University of Cambridge; South China Agricultural University; University of Southern Denmark; Palacky University Olomouc; Polish Academy of Sciences; Institute of Plant Genetics of the Polish Academy of Sciences; Czech Academy of Sciences; Palacky University Olomouc; Institute of Experimental Botany of the Czech Academy of Sciences; Palacky University Olomouc; University of Nottingham; Consejo Superior de Investigaciones Cientificas (CSIC); Universitat Politecnica de Valencia; CSIC-UPV - Instituto de Biologia Molecular y Celular de Plantas (IBMCP); University of Dundee; Ecole Normale Superieure de Lyon (ENS de LYON); Universite Lyon 1; Centre National de la Recherche Scientifique (CNRS); INRAE; North Carolina State University; North Carolina State University; Umea University; Swedish University of Agricultural Sciences; University of Cambridge; University of Cambridge; University of Tartu; Friedrich Miescher Institute for Biomedical Research; University of Cambridge
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.adx2867
• 发表日期: 2026-02-12
• 页码: 694-699
• 关键词: cryo-em; POLYAMINE OXIDASE; DWARF PHENOTYPE; ACL5 MUTANT; arabidopsis; rna; cytokinin; protein; expression; GROWTH

摘要

Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In Arabidopsis, the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SUPPRESSOR-OF-ACAULIS51 (SAC51) and SAC51-LIKEs (SACLs), which inhibit heterodimerization of the xylem development proteins LONESOME-HIGHWAY (LHW) and TARGET-OF-MONOPTEROS5. Here, we report a methyltransferase, OVERACHIEVER, that methylates the peptidyl transferase center of the 25S ribosomal RNA (rRNA). Residue m3U2952 promotes functional tSpm binding to a specific site connecting the P-site transfer RNA (tRNA) with rRNA residues in the peptidyl transferase center. This interaction enhances the translation of SACLs but inhibits that of LHW. Our study uncovers the dependency between a conserved rRNA base methylation and a polyamine in orchestrating cell fate decisions, highlighting a role for the ribosome chemical landscape in translational regulation.