A whole-brain single-cell atlas of circadian neural activity in mice

• 成果类型: Article
• 署名作者: Yamashita, Katsunari; Kinoshita, Fukuaki L.; Yoshida, Shota Y.; Matsumoto, Katsuhiko; Mitani, Tomoki T.; Fujishima, Hiroshi; Minami, Yoichi; Morii, Eiichi; Yamada, Rikuhiro G.; Okada, Seiji; Ueda, Hiroki R.
• 署名单位: RIKEN; University of Tokyo; University of Osaka; University of Osaka; University of Osaka; University of Osaka; University of Tokyo; Niigata University; Kurume University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.aea3381
• 发表日期: 2026-02-19
• 页码: eaea3381
• 关键词: suprachiasmatic nucleus; forebrain circuit; fos expression; BODY TIME; neurons; rhythm; sleep; mouse; rat; ORGANIZATION

摘要

The mammalian brain comprises numerous anatomical regions with distinct functions despite their extensive connectivity. How spontaneous neural activity is coordinated across regions over the circadian cycle remains elusive. We used tissue clearing and whole-brain c-Fos immunostaining on 144 mouse brains collected over 2 days under constant darkness. Time-series analysis revealed brainwide circadian rhythmicity at single-cell resolution, with 79% of the 642 anatomically defined regions oscillating in diverse circadian phases that delineate functional specializations. Voxelwise analyses further highlighted distinct subregions, suggesting intricate spatiotemporal coordination within regions. Additionally, brain circadian time could be accurately inferred from global c-Fos patterns using omics-derived prediction methods. This whole-brain circadian atlas enhances our understanding of neural coordination and provides a resource for integrating time-of-day information into functional and pharmacological research.