Nanodomain-localized formin gates symbiotic microbial entry in legume and solanaceous plants

• 成果类型: Article
• 署名作者: Qiao, Lijin; Sun, Heng; Tang, Jiping; Hernandez-Reyes, Casandra; Lace, Beatrice; Knerr, Julian; Schulze, Eija; Lee, Tak; Keller, Jean; Libourel, Cyril; Yao, Jilin; Zhao, Feiyang; Ni, Ying; Jia, Yutian; Xu, Xia; Yang, Guanghui; Zhang, Lin; Zhang, Yanli; Grosse, Robert; Tian, Changfu; Oldroyd, Giles E. D.; Delaux, Pierre-Marc; Ott, Thomas; Liang, Pengbo
• 署名单位: China Agricultural University; China Agricultural University; China Agricultural University; University of Freiburg; University of Freiburg; University of Freiburg; University of Cambridge; University of Cambridge; Centre National de la Recherche Scientifique (CNRS); Universite de Toulouse (EPE); Communaute d'universites et etablissements de Toulouse (Comue); China Agricultural University; Tsinghua University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.adx8542
• 发表日期: 2026-03-05
• 关键词: dependent protein-kinase; medicago-truncatula; actin cytoskeleton; NODULE DEVELOPMENT; plasma-membrane; ROOT HAIRS; arbuscule development; signal-transduction; nitrogen-fixation; GTPASE ROP6

摘要

Colonization of plant roots by symbionts requires substantial morphodynamic reorganization. Examples are actin-scaffolded microcompartments called infection pockets formed during root nodule symbiosis (RNS) by legumes. We demonstrate that the actin-binding formin SYFO2 is indispensable for rhizobial infection in Medicago truncatula, where it drives actin polymerization in phase-separated and symbiosis-specific nanodomains. SYFO2 also regulates symbiotically active arbuscules formed during mycorrhizal symbiosis in plants outside the nodulating clade, indicating that it was additionally recruited to promote rhizobial infections in legumes. As part of our aim to enable nitrogen fixation in nonlegumes, we activated endogenous SYFO2 by stably introducing the RNS master regulator NODULE INCEPTION (NIN) into the natural nonhost tomato. This demonstrates the possibility of recruiting arbuscular mycorrhizae-related genes into an engineered nodulation-specific pathway.