Spatial and morphological organization of mitochondria in neurons across a connectome

• 成果类型: Article
• 署名作者: Sager, Garrett; Pfeiffer, Paul; Wu, Heng; Pallasdies, Fabian; Gowers, Robert; Ravikumar, Snusha; Wu, Elizabeth; Colon-Ramos, Daniel; Schreiber, Susanne; Clark, Damon A.
• 署名单位: Yale University; Humboldt University of Berlin; Yale University; Yale University; Yale University; Yale University; Yale University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.ads6674
• 发表日期: 2026-03-19
• 页码: eads6674
• 关键词: DROSOPHILA MUSHROOM BODY; AXONAL MITOCHONDRIA; detecting neurons; transport; organelles; connectivity; compartments; homeostasis; movements; synapses

摘要

Neuronal function depends on mitochondria, but little is known about their organization across neurons. Using an electron microscopy Drosophila connectome, we uncovered quantitative rules governing the morphology and positioning of hundreds of thousands of mitochondria across thousands of neurons. We discovered that mitochondrial morphological features are specific to cell and neurotransmitter type, which provides fingerprints to identify neurons. Mitochondria are positioned with 2- to 3-micrometer precision relative to synaptic and structural features, with systematic differences across neuron types and compartments. Mitochondrial localization correlates with regional activity and postsynaptic targets. Analysis of a mouse visual cortex connectome confirms cell type-specific morphology and identifies partially divergent positioning rules. These results establish mitochondria as circuit-embedded organelles whose distribution links subcellular architecture to brain connectivity.