High-throughput single-cell omics using semipermeable capsules

• 成果类型: Article
• 署名作者: Baronas, Denis; Norvaisis, Simonas; Zvirblyte, Justina; Leonaviciene, Greta; Mikulenaite, Vincenta; Goda, Karolis; Kaseta, Vytautas; Sablauskas, Karolis; Griskevicius, Laimonas; Juzenas, Simonas; Mazutis, Linas
• 署名单位: Vilnius University; State Research Institute Centre for Innovative Medicine; Vilnius University; Vilnius University; Umea University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.ady7227
• 发表日期: 2026-03-12
• 关键词: messenger-rna; STATES; expression; hybridization

摘要

Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput single-cell omics methods and tools that efficiently probe the cellular phenotype and genotype. Here, we present a versatile technology based on semipermeable capsules (SPCs) tailored for a variety of high-throughput nucleic acid assays, including single-cell genome and mRNA sequencing, and fluorescence-activated cell sorting-based isolation of individual transcriptomes based on a nucleic acid marker of interest. Being biocompatible, the SPCs support single-cell cultivation and clonal expansion over long periods of time, thereby overcoming a fundamental limitation of droplet microfluidics platforms. Overall, the SPCs represent a customizable and broadly applicable tool for easy-to-use, scalable single-cell omics applications that are built on multistep biochemical reactions.