Family single-cell atlases reveal pig pregnancy and fetal growth restriction critical cell types

• 成果类型: Article
• 署名作者: Cai, Liping; Zhang, Qing; Yao, Tianxiong; Zou, Xiaoxiao; Xie, Lei; Yang, Siyu; Xiao, Yanyuan; Huang, Fei; Peng, Zhiwei; Yang, Jiawen; Huang, Jianzhen; Jing, Qiqi; Ling, Ziqi; Zheng, Min; Qiao, Chuanmin; Wu, Jinyuan; Chen, Jiaqi; Jiang, Tao; Liu, Qin; Liu, Min; Chen, Zhe; Li, Jingquan; Jiang, Hui; Jiang, Haoyun; Chen, Zuoquan; Liao, Yaya; Huang, Yizhong; Li, Longyun; Zhou, Yunyan; Zhang, Zhou; Yin, Chao; Ye, Yu; Yang, Hui; Chen, Biao; Wang, Zhong; Gong, Huanfa; Chen, Dong; Huang, Cong; Tong, Xinkai; Zhou, Zhimin; Fu, Hao; Sun, Yingchun; Sun, Xiao; Yan, Min; Wu, Lin; Xiong, Shuqi; Wang, Yaxiang; Xie, Xinke; Zhou, Mengqing; Liu, Xiaodong; Xie, Xianhua; Wu, Fusheng; Liu, Zifeng; Ge, Mei; Xiong, Sanya; Li, Chenyu; Shen, Tao; Liu, Yuxin; Tang, Xi; Chang, Xiaolong; Liu, Siyi; Yu, Chuangang; Yu, Naixiang; Hu, Zikang; Cui, Dengshuai; Zhong, Jiahong; Liu, Sha; Hu, Jianchao; Wen, Yang; Zhou, Weiwei; Wei, Guanyue; Lin, Shiyao; Xiahou, Yiwen; Chen, Liqing; Li, Jianjun; Liu, Ruirong; Chen, Xiaoyun; Shu, Yujie; Chen, Feng; Wei, Chao; Hu, Xiaofen; Li, Yong; Hou, Yong; Zeng, Qingjie; Gao, Jun; Su, Ying; Li, Jing; Ma, Junwu; Guo, Yuanmei; Chen, Congying; Xiao, Shijun; Ai, Huashui; Zhang, Zhiyan; Liu, Weiwei; Yang, Qiang; Xing, Yuyun; Rao, Lin; Yang, Bin; Huang, Lusheng
• 署名单位: Jiangxi Agricultural University; Ministry of Science and Technology, China
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.adu7265
• 发表日期: 2026-03-26
• 页码: eadu7265
• 关键词: reconstruction; identification; transcriptome; endothelium; metabolism; HEALTH

摘要

Although massive cell atlases are already available, it is still a challenge to obtain an atlas of all tissues within a single body, especially in fetuses and pregnant mothers. We present a transcriptomic atlas of 2.56 million single cells covering 115 and 119 tissues from one fetal pig and its pregnant mother, respectively. We found that a cluster of heart capillary endothelial cells with enhanced fatty acid transit capability was enriched for pregnancy but restored after gestation. We also deciphered that l-leucine transport insufficiency in the trophoblast causes fetal growth restriction by reducing a muscle type II myofiber subcluster. Our all-from-one strategy enabled the identification of tissue cell type-specific transcription factors and provided insights into pregnancy heart adaptation and fetal growth restriction.