Control of circadian muscle glucose metabolism through the BMAL1-HIF axis in obesity

• 成果类型: Article
• 署名作者: Chaikin, Claire A.; Thakkar, Abhishek, V; Steffeck, Adam W. T.; Pfrender, Eric M.; Hung, Kaitlyn; Zhu, Pei; Waldeck, Nathan J.; Nozawa, Rino; Song, Weimin; Futtner, Christopher R.; Quattrocelli, Mattia; Bass, Joseph; Ben-Sahra, Issam; Peek, Clara B.
• 署名单位: Northwestern University; Feinberg School of Medicine; Northwestern University; Feinberg School of Medicine; Cincinnati Children's Hospital Medical Center; University System of Ohio; University of Cincinnati
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-15291
• DOI: 10.1073/pnas.2424046122
• 发表日期: 2025-04-01
• 关键词: skeletal-muscle; hypoxic response; food-intake; shift work; clock; pfkfb3; gene; time; expression; protein

摘要

Disruptions of circadian rhythms are widespread in modern society and lead to accelerated and worsened symptoms of metabolic syndrome. In healthy mice, the circadian clock factor BMAL1 is required for skeletal muscle function and metabolism. However, the importance of muscle BMAL1 in the development of metabolic diseases, such as diet- induced obesity (DIO), remains unclear. Here, we demonstrate that skeletal muscle-specific BMAL1- deficient mice exhibit worsened glucose tolerance upon high- fat diet feeding, despite no evidence of increased weight gain. Metabolite profiling from Bmal1- deficient muscles revealed impaired glucose utilization specifically at early steps in glycolysis that dictate the switch between anabolic and catabolic glucose fate. We provide evidence that this is due to abnormal control of the nutrient stress-responsive hypoxia- inducible factor glucose tolerance and expression of 217/736 dysregulated genes during DIO, including glycolytic enzymes. Together, these data indicate that during DIO, skeletal muscle BMAL1 ences the development of high- fat- diet- induced glucose intolerance.