Salmonella produces sulfide to compete with Escherichia coli in the gut lumen

• 成果类型: Article
• 署名作者: Larabi, Anais B.; Tiffany, Connor R.; Masson, Hugo L. P.; Nguyen, Henry; Bejarano, Eli J.; Liou, Megan J.; Radlinski, Lauren C.; Demars, Aurore M.; Tsolis, Renee M.; Baumler, Andreas J.
• 署名单位: University of California System; University of California Davis; University of Pennsylvania; University of Pennsylvania; Pennsylvania Medicine; Childrens Hospital of Philadelphia; University of Pennsylvania
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14973
• DOI: 10.1073/pnas.2504095122
• 发表日期: 2025-09-16
• 关键词: enterica serovar typhimurium; hydrogen-sulfide; serotype typhimurium; sequence-analysis; identification; genes; methanethiol; inflammation; desulfomonas; clostridium

摘要

Hydrogen sulfide production is a characteristic that distinguishes Salmonella serovars from closely related species, such as Escherichia coli, but its biological significance remains obscure. Here, we show that PhsABC and AsrABC-mediated hydrogen sulfide production by Salmonella enterica serovar (S.) Typhimurium was linked to an increased abundance of Deltaproteobacteria and inhibition of cytochrome bd oxidase-mediated aerobic respiration of Escherichia coli in the murine large intestine. Functional phsABC and asrABC operons provided a growth benefit to S. Typhimurium in the cecum of Enterobacterales-free conventional mice only upon inoculation with commensal E. coli. In gnotobiotic mice engrafted with a defined community of 17 human Clostridia isolates, S. Typhimurium infection inhibited cytochrome bd oxidase-mediated aerobic respiration in E. coli only in the presence of Desulfovibrio piger, a sulfide-producing representative of the Deltaproteobacteria. A S. Typhimurium strain deficient for hydrogen sulfide production (phsA asrA mutant) did not inhibit cytochrome bd oxidase-mediated aerobic respiration in E. coli, even when D. piger was present. Collectively, these data suggest that the phsABC and asrABC operons of Salmonella serovars provide a benefit during competition with closely related bacteria, such as E. coli, by inhibiting cytochrome bd oxidase-mediated aerobic respiration of the commensal.