The Arabidopsis demethylase REF6 physically interacts with phyB to promote hypocotyl elongation under red light

• 成果类型: Article
• 署名作者: Yan, Yan; Zhu, Jiaping; Qiu, Qi; Li, Jigang; Cao, Xiaofeng; Deng, Xian
• 署名单位: Chinese Academy of Sciences; Institute of Genetics & Developmental Biology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; China Agricultural University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14797
• DOI: 10.1073/pnas.2417253122
• 发表日期: 2025-03-18
• 关键词: histone deacetylase hda15; transcription factor; seed-germination; induced phosphorylation; negative regulator; cell elongation; phytochrome; degradation; gene; photomorphogenesis

摘要

The plant photoreceptor phytochrome B (phyB) mediates the responses of plants to red (R) light. Trimethylation of histone H3 at Lys27 (H3K27me3) plays a crucial role in governing gene expression and controlling the response of plants to environmental changes. However, how dynamic H3K27me3 mediates plant response to R light is poorly understood. Here, we report that RELATIVE OF EARLY FLOWERING 6 (REF6), an H3K27me3 demethylase, promotes hypocotyl elongation under R light in Arabidopsis. Upon exposure to R light, REF6 preferentially interacts with the active Pfr form of phyB. Consequently, phyB enhances REF6 accumulation and its binding ability, which are necessary for inducing cell- elongation- related genes from open chromatin, ensuring normal plant growth under prolonged light exposure. Moreover, REF6 acts together with the phyB- PIF4 module to mediate light regulation of hypocotyl growth. These findings provide insights into the understanding of how phytochromes, epigenetic factors, and transcription factors coordinately control plant growth in response to changing light environment.