Remote analysis and management of sweat biomarkers using a wearable microfluidic sticker in adult cystic fibrosis patients

• 成果类型: Article
• 署名作者: Nelson, Rachel S.; Grossman, Margaret G.; Klug, Zasu M.; Calamari, Mia; Donayre, Alvaro; Cybulski, Thaddeus; Schooley, Jared; Griffith, Garett J.; Corcos, Daniel M.; Wright, Donald E.; Wallace, Jessica C.; Yang, Da Som; Wright Jr, John A.; Rogers, John A.; Lee, Stephen P.; Ghaffari, Roozbeh; Aranyosi, A. J.; Jain, Manu
• 署名单位: Northwestern University; Feinberg School of Medicine; Northwestern University; Northwestern University; Chung Ang University; Northwestern University; Northwestern University; Northwestern University; US Department of Veterans Affairs; Veterans Health Administration (VHA); Jesse Brown VA Medical Center; Northwestern University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14021
• DOI: 10.1073/pnas.2506137122
• 发表日期: 2025-08-19
• 关键词: exercise; chloride; patch

摘要

Sweat parameters such as volume and chloride concentration may offer invaluable clinical insights for people with CF (PwCF). Pilocarpine-induced sweat collection for chloridometry measurement is the gold standard for a CF diagnosis, but this technique is cumbersome and not suitable for remote settings or repeat measurements. We have previously reported the utility of a skin-interfaced microfluidic device (CF Patch) in conjunction with a smartphone image processing platform that enables real-time measurement of sweating rates and sodium chloride loss in laboratory and remote settings. Here, we conducted clinical studies assessing the accuracy of the CF Patch compared to chloridometry when using pilocarpine to induce sweat. We also tested the feasibility and accuracy of exercise-induced sweat chloride measurements in PwCF and healthy volunteers (HV). In the laboratory, using either pilocarpine or exercise to induce sweat, the CF Patch demonstrated strong correlations with sweat chloride measured by pilocarpine-induced chloridometry. In remote settings, exercise-induced sweat chlorides measured using the CF patch were strongly correlated with in-laboratory exercise-induced CF patch sweat chlorides in HV but had a weaker correlation in PwCF. For PwCF on CFTR modulators, there was greater day-to-day variability in sweat chloride compared to HV, which highlights the limitations of assessing CFTR modulator efficacy and pharmacodynamics based on a single in-laboratory chloridometry measurement. Moreover, these findings demonstrate that the CF Patch is suitable as a remote management device capable of measuring serial sweat chloride concentrations and offers the potential of monitoring the efficacy of CF medication regimens but should not replace pilocarpine-based chloridometry for making a CF diagnosis.