Structural basis for Rad54-and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination

• 成果类型: Article
• 署名作者: Shin, Yeonoh; Petassi, Michael T.; Jessop, Aidan M.; Kim, Stefan Y.; Matei, Razvan; Morse, Katherine; Raina, Vivek B.; Roy, Upasana; Greene, Eric C.
• 署名单位: New York University; Columbia University; NewYork-Presbyterian Hospital
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14013
• DOI: 10.1073/pnas.2510007122
• 发表日期: 2025-09-16
• 关键词: homologous recombination; saccharomyces-cerevisiae; branch migration; yeast rad51; x-ray; dna; protein; dmc1; repair; chromatin

摘要

Rad51 catalyzes the DNA pairing reactions that take place during homologous recombination (HR), and HR must be tightly regulated to ensure physiologically appropriate outcomes. Rad54 is an ATP- dependent DNA motor protein that stimulates Rad51 activity during mitosis. In meiosis Rad51 is downregulated by the protein Hed1, which blocks Rad54 binding to Rad51, and allows Dmc1 to function as the active recombinase. We currently have a poor understanding of the regulatory interplay between Rad54, Rad54, and we solve a CryoEM structure of this motif bound to Rad51. We also identify sister chromatids during mitosis and how Rad51 is downregulated by Hed1 upon entry between homologous chromosomes.