Phased ERK responsiveness and developmental robustness regulate teleost skin morphogenesis

• 成果类型: Article
• 署名作者: Ramkumar, Nitya; Richardson, Christian; O'Brien, Makinnon; Butt, Faraz Ahmed; Park, Jieun; Chao, Anna T.; Bagnat, Michel; Poss, Kenneth D.; Di Talia, Stefano
• 署名单位: Duke University; Duke University; Duke University; University of Wisconsin System; University of Wisconsin Madison; The Morgridge Institute for Research, Inc.; University of Wisconsin System; University of Wisconsin Madison; Duke University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13839
• DOI: 10.1073/pnas.2410430122
• 发表日期: 2025-03-11
• 关键词: cell-division; zebrafish; proliferation; GROWTH; intercalation; extension; epidermis; DYNAMICS; genes; size

摘要

Elongation of the vertebrate embryonic axis necessitates rapid expansion of the epidermis to accommodate the growth of underlying tissues. Here, we generated a toolkit to visualize and quantify signaling in entire cell populations of the periderm, the outermost layer of the epidermis, in live developing zebrafish. We find that oriented cell divisions facilitate growth of the early periderm during axial elongation rather than cell addition from the basal layer. Activity levels of Extracellular signal- regulated kinase (ERK), a downstream effector of the MAPK pathway, gauged by a live biosensor, predict cell cycle entry, and optogenetic ERK activation regulates cell cycling dynamics. As ity becomes more pulsatile and functionally transitions to promote hypertrophic cell growth. Targeted genetic blockade of cell division generates animals with oversized ings reveal stage- dependent differential responsiveness to ERK signaling and marked developmental robustness in growing teleost skin.