A self- assembling surface layer flattens the cytokinetic furrow to aid cell division in an archaeon

• 成果类型: Article
• 署名作者: Foo, Sherman; Caspy, Ido; Cezanne, Alice; Bharat, Tanmay A. M.; Baum, Buzz
• 署名单位: MRC Laboratory Molecular Biology; UK Research & Innovation (UKRI); Medical Research Council UK (MRC); UK Research & Innovation (UKRI); Medical Research Council UK (MRC); MRC Laboratory Molecular Biology
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13805
• DOI: 10.1073/pnas.2501044122
• 发表日期: 2025-06-18
• 关键词: escrt-iii homologs; s-layer; protein; sulfolobus; CURVATURE; TOPOLOGY; genus

摘要

The surface layer or S- layer is a two- dimensional lattice of proteins that coats a wide are thought to play an important role in chemically and physically insulating cells from the external environment. Here, we show that the integrity of the S- layer in Sulfolobus acidocaldarius is maintained as cells grow via a process of self- assembly as SlaA monomers fill gaps in the lattice. Although this lattice which is physically tethered to the membrane might be expected to hinder cell division, we show that the S- layer flattens the membrane at cytokinesis to accelerate ESCRT- III- dependent cell division-and is important for robust, successful cell divisions under conditions of mechanical stress. Taken together, these results define the rules governing S- layer self- assembly and show how a flexible lattice coat that is coupled to the underlying membrane can both provide a cell with mechanical support and help to drive rapid and functionally important changes in cell shape.