RNA sequencing analysis of viromes of Aedes albopictus and Aedes vexans collected from NEON sites

• 成果类型: Article
• 署名作者: Paull, Sara H.; Spurbeck, Rachel R.; Hasan, Nur A.; Brumfield, Kyle D.; Catlin, Lindsay A.; Netherland Jr, Michael J.; Smith, Anthony K.; Thibault, Katherine M.; Colwell, Rita R.
• 署名单位: Battelle Memorial Institute; Battelle Memorial Institute
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13351
• DOI: 10.1073/pnas.2403591122
• 发表日期: 2025-05-20
• 关键词: insect-specific viruses; mosquito; DISCOVERY; alignment; vectors; aegypti

摘要

Climate change is significantly impacting the geographic range of many animal species and their associated microorganisms, hence influencing emergence of vector- borne diseases. Mosquito- borne viruses represent a potential major reservoir of human pathogens, highlighting the need for improved understanding of ecological factors associated with variation in the mosquito viral community (virome). Here, a subtractive hybridization method coupled with RNAseq of individual mosquito specimens was used to profile RNA viromes of individual co- occurring Aedes albopictus and Aedes vexans mosquitoes across a 2,000 km spatial scale. Samples were collected and archived by the National suggest that mosquito species are an important factor in RNA viral community composition. Significantly higher viral diversity was detected in A. albopictus compared to A.vexans. However, season, year, and site of sample collection did not show strong association with virome profiles, supporting the hypothesis that factors unique to the mosquito host species (e.g., larval habitat or vector competence) influence the structure of mosquito viromes.