RACK1A positively regulates opening of the apical hook in Arabidopsis thaliana via suppression of its auxin response gradient

• 成果类型: Article
• 署名作者: Ma, Qian; Liu, Sijia; Doyle, Siamsa M.; Raggi, Sara; Parizkova, Barbora; Barange, Deepak Kumar; Ratnakaram, Hemamshu; Wilkinson, Edward G.; Garcia, Isidro Crespo; Bygdell, Joakim; Wingsle, Gunnar; Boer, Dirk Roeland; Strader, Lucia C.; Almqvist, Fredrik; Novak, Ondrej; Robert, Stephanie
• 署名单位: Swedish University of Agricultural Sciences; Umea University; Palacky University Olomouc; Czech Academy of Sciences; Institute of Experimental Botany of the Czech Academy of Sciences; Umea University; Duke University; Umea University; Ghent University; Flanders Institute for Biotechnology (VIB); Shenyang Agricultural University; Swedish University of Agricultural Sciences; Umea University; Colorado State University System; Colorado State University Fort Collins
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13322
• DOI: 10.1073/pnas.2407224122
• 发表日期: 2025-07-29
• 关键词: differential growth; rational design; protein; antagonist; encodes; screen; FAMILY; rub1

摘要

Apical hook development is an ideal model for studying differential growth in plants and is controlled by complex phytohormonal crosstalk, with auxin being the major player. Here, we identified a bioactive small molecule that decelerates apical hook opening in Arabidopsis thaliana. Our genetic studies suggest that this molecule enhances or maintains the auxin maximum found in the inner hook side and requires certain auxin signaling components to direct target of this compound. We present data in support of RACK1A playing a positive role in apical hook opening by activating specific auxin signaling mechanisms and negatively regulating the differential auxin response gradient across the hook, thereby adjusting differential cell growth, an essential process for organ structure and function in plants.