Two transcription factors play critical roles in mediating epigenetic regulation of fruit ripening in tomato

• 成果类型: Article
• 署名作者: Niu, Qingfeng; Xu, Yaping; Huang, Huan; Li, Linzhu; Tang, Dengguo; Wu, Siqun; Liu, Ping; Liu, Ruie; Ma, Yu; Zhang, Bo; Zhu, Jian-Kang; Lang, Zhaobo
• 署名单位: Anhui Agricultural University; Southern University of Science & Technology; Southern University of Science & Technology; Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS; Chinese Academy of Sciences; Shanghai Institute of Nutrition & Health, CAS; Zhejiang University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12715
• DOI: 10.1073/pnas.24227981221
• 发表日期: 2025-04-15
• 关键词: dna methylation; cpg methylation; gene; homologs; proteins; binding; complexes; epigenome; DYNAMICS

摘要

DNA methylation regulates fruit ripening in tomato, and disruption of the DNA ylation and impaired ripening. We report here that the transcription factors Ripening Inhibitor (RIN) and FRUITFULL 1 (FUL1) play critical roles in mediating the effect of DNA methylation on tomato fruit ripening. RIN and FUL1 are silenced in dml2 mutant plants, and the defective ripening phenotype of dml2 is mimicked by the rin/ ful1 double mutant. Restoration of RIN expression in dml2 partially rescues its ripening defects. DNA methylation controls ripening not only by regulating the expression of RIN and FUL1 but also by interfering with the genomic binding of RIN. In dml2 mutant plants, RIN cannot bind to some of its targets in vivo even though DNA methylation does not interfere with RIN binding in vitro; this inhibited binding in vivo is correlated with increased DNA methylation and histone H3 enrichment within 100 bp of the binding site. Our work uncovers the molecular mechanisms underlying DNA methylation control of fruit ripening in tomato.