A pentatomomorpha- specific salivary protein activates plant immunity and is critical for insect feeding

• 成果类型: Article
• 署名作者: Hu, Biao; Feng, Xiuli; Xu, Manru; Huang, Yue; Guo, Chunyun; Yuan, Ruikun; Li, Yiyuan; Wei, Zhongyan; Chen, Jianping; Sun, Zongtao
• 署名单位: Ningbo University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11521
• DOI: 10.1073/pnas.2425190122
• 发表日期: 2025-02-04
• 关键词: riptortus-pedestris; perception; resistance; hsp90; defenses; hsp70; hsp40

摘要

The stinkbug Riptortus pedestris, notorious for inducing soybean staygreen-like syndrome, employs a range of salivary proteins to manipulate the host plant for its benefit. Here, we show that RpSP1, a salivary protein specific to Pentatomomorpha, triggers plant defense responses in multiple plant species. RpSP1 interacts with and stabilizes a HSP40 family protein GmSPIP1 and is dependent on GmSPIP1 to induce cell death. We show that a critical 22-amino acid peptide within RpSP1 acts as an intracellular insect-derived elicitor. Furthermore, RpSP1 enhances insect-feeding efficiency. The dual functionality of RpSP1 is highlighted by the significant reduction of soybean staygreen-like syndrome following its overexpression in soybean plants or knockdown in insects. Our findings elucidate the complex molecular interactions between plants and herbivores, positioning RpSP1 as a crucial target for developing advanced pest management strategies with broad implications for agricultural biology.