Environmental resistome-guided development of resistance-tolerant antibiotics

• 成果类型: Article
• 署名作者: Peek, James; Bhattacharjee, Abir; Burian, Jan; Hsieh, David Chun- Cheng; Hernandez, Yozen; Ternei, Melinda; Panfil, Cecilia; Brady, Sean F.
• 署名单位: Rockefeller University; Rockefeller University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11479
• DOI: 10.1073/pnas.2504781122
• 发表日期: 2025-05-19
• 关键词: beta-lactamases; xanthomonas-albilineans; albicidin resistance; mass-spectrometry; escherichia-coli; inhibitor; protein; gene

摘要

Failure to anticipate new forms of antibiotic resistance has led to resistance developing rapidly to virtually all antibiotics that have entered clinical use. Many of the most problematic types of resistance originated in the environment, where ancient arms races between antibiotic-producing microbes and their competitors have created vast arsenals of antibiotics and resistance. Seizing on the knowledge that resistance in nature is frequently a harbinger of future clinical resistance, we propose introducing an additional step into the antibiotic development process that exploits the susceptibility of development candidates to environmental resistance as a metric for prioritizing lead compounds and as a roadmap for their structural optimization. Using the antibiotic albicidin as a model, we show how the environmental resistome can guide the development of more resistance-tolerant leads. We used metagenomic surveys to identify resistance vulnerabilities for albicidin and guide the synthesis of analogs that evade the resistance threats. We found that natural albicidin analogs (congeners) were especially enriched in structural features that escape resistance, which inspired our syntheses and provided compelling evidence for the evolution of families of antibiotics in response to resistance in nature. The coupling of metagenomics-based resistance surveillance with structural optimizations of new antibiotics is a broadly applicable approach that is easily integrated into antibiotic development programs to generate compounds that are more resilient in the face of resistance.