Rolling vesicles: From confined rotational flows to surface-enabled motion

• 成果类型: Article
• 署名作者: Magrinya, Paula; Palacios-Alonso, Pablo; Llombart, Pablo; Delgado-Buscalioni, Rafael; Alexander-Katz, Alfredo; Arriaga, Laura R.; Aragones, Juan L.
• 署名单位: Autonomous University of Madrid; Massachusetts Institute of Technology (MIT)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11280
• DOI: 10.1073/pnas.2424236122
• 发表日期: 2025-03-25
• 关键词: slow viscous motion; polymersomes; adhesion; SEPARATION; wall

摘要

Friction forces are essential for cell movement, yet they also trigger numerous active cellular responses, complicating their measurement in vivo. Here, we introduce a synthetic model designed to measure friction forces between biomimetic membranes and substrates. The model consists of a vesicle with precisely controlled properties, fabricated via microfluidics, encapsulating a single ferromagnetic particle that is magnetically driven to rotate. The rotation of the particle generates a confined rotational flow, setting the vesicle membrane into motion. By adjusting the magnetic field frequency and vesicle size, the rotation frequency of the vesicle can be finely controlled, resulting in a rolling vesicle that functions as an effective tribological tool across a wide frequency range. At low frequencies, molecular contact between the membrane and substrate dominates frictional interactions, which enables determination of the contact the vesicles to slip rather than roll. Adjusting membrane fluidity and incorporating specific ligand-receptor interactions within this model will enable detailed studies of frictional forces in more complex biomimetic systems, providing key insights into the mechanisms of cell movement and mechanotransduction.