A plant CLE peptide and its fungal mimic promote arbuscular mycorrhizal symbiosis via CRN-mediated ROS suppression

• 成果类型: Article
• 署名作者: Bashyal, Sagar; Everett, Hasani; Matsuura, Suzanne; Muller, Lena Maria
• 署名单位: Salk Institute; University of California System; University of California San Diego; University of Miami
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11037
• DOI: 10.1073/pnas.2422215122
• 发表日期: 2025-04-22
• 关键词: parasitic nematode; signaling pathways; lotus-japonicus; nodule number; gene; colonization; clavata2; roots; cell; autoregulation

摘要

CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) peptides have emerged as key regulators of plant-microbe interactions, including arbuscular mycorrhizal (AM) symbiosis. Here, we identify Medicago truncatula CLE16 as a positive regulator of AM symbiosis. MtCLE16is expressed in root cells colonized by AM fungi (AMF) and its overexpression within colonized tissues increases arbuscule abundance by finetuning their growth and lifespan. Functional and transcriptomic analyses reveal that MtCLE16 acts via the M. truncatula pseudokinase CORYNE (MtCRN) and suppresses the accumulation of reactive oxygen species (ROS) in roots, thereby attenuating immune responses and promoting root colonization by mutualistic AM fungi. Notably, AMF also express MtCLE16-like peptides. We show that the Rhizophagus irregularis MtCLE16-like peptide, RiCLE1, also attenuates ROS and promotes AMF colonization via MtCRN. This finding suggests that RiCLE1 can interfere with the MtCLE16-MtCRN signaling module of host roots to benefit the fungus. Our research uncovers a functional mechanism underpinning cross-kingdom signaling and molecular mimicry in mutualistic plant-microbe interactions.