A conserved editing mechanism for the fidelity of bacterial cell wall biosynthesis

• 成果类型: Article
• 署名作者: Garde, Shambhavi; Selvaraj, Harikrishnan; Chandramouli, Aakash; Reddy, Gundlapally S.; Bahety, Devesh; Chodisetti, Pavan Kumar; Kamat, Siddhesh S.; Reddy, Manjula
• 署名单位: Council of Scientific & Industrial Research (CSIR) - India; CSIR - Centre for Cellular & Molecular Biology (CCMB); Indian Institute of Science Education & Research (IISER) Pune; Barcelona Institute of Science & Technology; Pompeu Fabra University; Centre de Regulacio Genomica (CRG); Scripps Research Institute
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10994
• DOI: 10.1073/pnas.2505676122
• 发表日期: 2025-07-15
• 关键词: purine nucleoside phosphorylase; l-alanine ligase; escherichia-coli; cytoplasmic steps; sp nov.; purification; serine; RECOGNITION; precursors; disease

摘要

Peptidoglycan (PG), a defining feature of the bacterial cell envelope, is crucial for cell integrity and morphology. PG is a macromolecular mesh consisting of glycan polymers crosslinked by short peptides encasing the cytoplasmic membrane. PG peptides contain two to five amino acids of both L- and D- configuration, with a conserved L- alanine residue at the first position in most bacteria. We previously identified a beta- lactam hypersensitive mutant of Escherichia coli lacking yfiH (renamed pgeF) that shows incorporation of L- serine or glycine instead of L- alanine. Here, we demonstrate that PgeF is an editing enzyme that imparts fidelity to the biosynthesis of PG peptides across bacterial genera. Using extensive mass spectrometry, we find accumulation of misincorporated cytoplasmic PG precursors in the absence of pgeF. Detailed biochemical analysis of several bacterial PgeF homologs reveals that PgeF specifically cleaves serine or glycine but not alanine from the PG precursors. Additionally, expression of heterologous ligases that incorporate L- serine or glycine is lethal in the absence of pgeF, indicating that L- alanine at the first position is crucial for wall integrity. Interestingly, PgeF is selectively conserved in bacteria and vertebrates; however, we find that the PG editing activity is exclusive to bacterial homologs. Furthermore, homologs from both taxa were previously characterized as purine nucleoside phosphorylases relevance in bacterial systems. Overall, our study demonstrates the existence of a conserved proofreading pathway that is fundamental to the integrity of the bacterial cell wall.