Bacillus subtilis 6A and Escherichia coli 670 lacking 6 region 1.1 are not released during transcription initiation and elongation

• 成果类型: Article
• 署名作者: Tewary, Aniruddha; Sengupta, Shreya; Mukherjee, Soumya; Hazra, Nilanjana; Ebright, Yon W.; Ebright, Richard H.; Mukhopadhyay, Jayanta
• 署名单位: Department of Science & Technology (India); Bose Institute; Rutgers University System; Rutgers University New Brunswick; Rutgers University System; Rutgers University New Brunswick; University System of Ohio; University of Toledo; University System of Ohio; University of Toledo
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10520
• DOI: 10.1073/pnas.2503801122
• 发表日期: 2025-09-23
• 关键词: rna-polymerase holoenzyme; sigma(70) subunit; dna; binding; retention; assay

摘要

A 6 cycle in which the initiation factor 6 associates with RNA polymerase (RNAP) core enzyme to permit transcription initiation and dissociates from RNAP core enzyme to permit transcription elongation, has been proposed to occur and to be an essential step for 6-exchange, with all principal 6 factors from all bacteria. These proposals were based on studies of the principal 6 factor of Escherichia coli, 670, which generally, albeit not obligatorily, is released from RNAP upon the transition from transcription initiation to elongation. Here, we show that, in contrast to E. coli 670 , the Bacillus subtilis principal 6 factor, 6A, is not released and is retained on RNAP core throughout transcription elongation. We further show that a mutant E. coli 670 derivative lacking 6 region 1.1 (6 R1.1) is not released and is retained on RNAP core throughout transcription elongation. We also observe that B. subtilis 6A and the mutant E. coli 670 derivative lacking 6 R1.1 interact much more stably with RNAP than full-length E. coli 670. Our results indicate that the 6 cycle is not a universal phenomenon in bacteria.