WT1 directs normal progesterone receptor-chromatin binding essential for uterine receptivity at peri- implantation

• 成果类型: Article
• 署名作者: Ni, Zhangli; Guo, Chuanhui; Liu, Xiaojing; Li, Gaizhen; Tang, Yedong; Cai, Han; Huang, Weiyu; Gao, Fei; Tu, Zhaowei; Wang, Chao; Wang, Haibin; Kong, Shuangbo; Bao, Haili
• 署名单位: Xiamen University; China Agricultural University; Chinese Academy of Sciences; Institute of Zoology, CAS; Guangzhou Medical University; Xiamen University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9869
• DOI: 10.1073/pnas.2504361122/-/DCSupplemental
• 发表日期: 2025-07-15
• 关键词: endometrial stromal cells; suppressor gene wt1; embryo implantation; indian hedgehog; coup-tfii; transcription; expression; decidualization; responsiveness; coactivator-2

摘要

Progesterone receptor (PR)- mediated progesterone (P4) signaling plays a crucial role in the establishment of uterine receptivity which is the prerequisite for successful embryo implantation in mammals. However, detailed molecular mechanisms underlying PR-chromatin binding and transcriptional activity in the uterus remain largely elusive. Here, combining the P4- administrated ovariectomized mouse model and PR-chromatin immunoprecipitation sequencing, we identified transcription factor WT1 as a poten-tial cooperator of PR in the uterus. WT1 was specifically expressed in uterine stromal cells. Uterine deletion of Wt1 resulted in implantation failure due to attenuated P4 responsiveness in stromal cells and aberrant uterine receptivity. Mechanistically, WT1 physically interacted with PR in stromal cells, and exhibited genome-wide co- occupancy with PR. Furthermore, WT1 was indispensable for directing PR onto the chromatin via corecruitment and tethering manners, and facilitated PR to activate the transcription of target genes that were critical for uterine receptivity. Collectively, our study provided substantial evidence that WT1 served as a functional partner of PR in uterine stromal cells, thus guaranteeing P4 responsiveness conducive to uterine receptivity