Antiviral Mx proteins have an ancient origin and widespread distribution among eukaryotes
成果类型：
Article
署名作者：
Langley, Caroline A.; Dietzen, Peter A.; Emerman, Michael; Tenthorey, Jeannette L.; Malik, Harmit S.
署名单位：
University of Washington; University of Washington Seattle; Fred Hutchinson Cancer Center; Fred Hutchinson Cancer Center; University of California System; University of California San Francisco; Howard Hughes Medical Institute; Fred Hutchinson Cancer Center
刊物名称：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN/ISSBN：
0027-9470
DOI：
10.1073/pnas.2416811122
发表日期：
2025-01-24
关键词：
dynamin-like protein
mitochondrial fusion
chloroplast division
peroxisome abundance
structural insights
mammalian homologs
membrane fission
gtp hydrolysis
virus
opa1
摘要：
Mx proteins, first identified in mammals, encode potent antiviral activity against a wide range of viruses. Mx proteins arose within the Dynamin superfamily of proteins (DSP), which mediate critical cellular processes, such as endocytosis and mitochondrial, plastid, and peroxisomal dynamics. Despite their crucial role, the evolutionary origins of Mx proteins are poorly understood. Through comprehensive phylogenomic analyses with progressively expanded taxonomic sampling, we demonstrate that Mx proteins predate the interferon signaling system in vertebrates. Our analyses find an ancient monophyletic DSP lineage in eukaryotes that groups vertebrate and invertebrate Mx proteins with fungal MxF proteins, the largely uncharacterized plant and algal Dynamin 4A/4C proteins, and representatives from several other eukaryotic lineages, suggesting that Mx- like proteins date back close to the origin of Eukarya. Our phylogenetic analyses also find host- encoded and nucleocytoplasmic large DNA viruses- encoded DSPs interspersed in four distinct DSP lineages, indicating recurrent viral theft of host DSPs. Our analyses thus reveal an ancient history of viral and antiviral functions encoded by the Dynamin superfamily in eukaryotes.