tRNA selectivity during ribosome- associated quality control regulates the critical sterility- inducing temperature in two- line hybrid rice

• 成果类型: Article
• 署名作者: Zhou, Can; Liu, Chunyan; Yan, Bin; Sun, Jing; Li, Shengdong; Li, Ji; Wang, Jia; Huang, Xiahe; Yan, Wei; Yang, Shuying; Fu, Chenjian; Qin, Peng; Fu, Xingxue; Zhao, Xinghui; Wu, Yaxian; Song, Xianwei; Wang, Yingchun; Qian, Wenfeng; Yang, Yuanzhu; Cao, Xiaofeng
• 署名单位: Chinese Academy of Sciences; Institute of Genetics & Developmental Biology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Beijing Academy of Agriculture & Forestry Sciences (BAAFS); Southern University of Science & Technology
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9238
• DOI: 10.1073/pnas.2417526122
• 发表日期: 2025-02-11
• 关键词: read alignment; translation; degradation; elongation; mechanism; rqc

摘要

The two- line hybrid rice system, a cutting- edge hybrid rice breeding technology, has greatly boosted global food security. In thermo- sensitive genic male sterile (TGMS) lines, the critical sterility- inducing temperature (CSIT; the temperature at which TGMS lines change from male fertile to complete male sterile) acts as a key threshold. We recently uncovered that thermo- sensitive genic male sterility 5 (tms5), a sterile locus presenting in over 95% of TGMS lines, leads to the overaccumulation of 2 ',3 '- cyclic phosphate (cP)-OCCA-tRNAs and a deficiency of mature tRNAs, which underlies the molecular mechanism of tms5- mediated TGMS. However, there are a few reports on the regulatory mechanism controlling CSIT. Here, we identified a suppressor of tms5, an amino acid substitution (T552I) in the rice Rqc2 (ribosome- associated quality control 2), increases the CSIT in tms5 lines through its C- terminal alanine and threonine modification (CATylation) activity.This substitution alters tRNA selectivity, leading to the recruitment of different tRNAs to the A- site of ribosome and CATylation rate by OsRqc2 during ribosome- associated quality control (RQC), a process that rescues stalled ribosomes and degrades abnormal nascent chains during translational elongation. Further, the mutation restores the levels of mature tRNA-Ser/Ile to increase the CSIT of tms5 lines. Our findings reveal the origin of overaccumulated cP-OCCA-tRNAs in tms5 lines, further deepening our understanding of the regulatory network in governing CSIT of TGMS lines containing tms5.