Stereospecific control of microbial growth by a combinatoric suite of chiral siderophores

• 成果类型: Article
• 署名作者: Stow, Parker R.; Forsch, Kiefer O.; Thomsen, Emil; Naka, Hiroaki; Haygood, Margo G.; Barbeau, Katherine A.; Butler, Alison
• 署名单位: University of California System; University of California Santa Barbara; University of California System; University of California San Diego; Scripps Institution of Oceanography; Utah System of Higher Education; University of Utah
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9228
• DOI: 10.1073/pnas.2423730122
• 发表日期: 2025-03-11
• 关键词: ferric enterobactin; optical antipode; iron uptake; pyochelin; identification; vanchrobactin; biosynthesis; transporters; rhizoferrin; RECOGNITION

摘要

Bacteria compete for iron by producing small-molecule chelators known as siderophores. The triscatechol siderophores trivanchrobactin and ruckerbactin, produced by Vibrio campbellii DS40M4 and Yersinia ruckeri YRB, respectively, are naturally occurring diastereomers that form chiral ferric complexes in opposing enantiomeric configurations. Chiral recognition is a hallmark of specificity in biological systems, yet the biological consequences of chiral coordination compounds are relatively unexplored. We demonstrate stereoselective discrimination of microbial growth and iron uptake by chiral Fe(III)-siderophores. The siderophore utilization pathway in V. campbellii DS40M4 is stereoselective for Lambda- Fe(III)-trivanchrobactin, but not the mismatched Delta- Fe(III)-ruckerbactin diastereomer. Chiral recognition is likely conferred by the stereospecificity of both the outer membrane receptor (OMR) protein FvtA and the periplasmic binding protein (PBP) FvtB, both of which must interact preferentially with the Lambda- configured Fe(III)- coordination complexes.