Large-scale combination screens reveal small-molecule sensitization of antibiotic-resistant gram-negative ESKAPE pathogens

• 成果类型: Article
• 署名作者: Tse, Megan W.; Zhu, Meilin; Peters, Benjamin; Hamami, Efrat; Chen, Julie; Davis, Kathleen P.; Nitz, Samuel; Weller, Juliane; Warrier, Thulasi; Hunt, Diana K.; Morales, Yoelkys; Kawate, Tomohiko; Gaulin, Jeffrey L.; Come, Jon H.; Hernandez-Bird, Juan; Huo, Wenwen; Neisewander, Isabelle; Yu, Xiao; Aceves-Salvador, Jose A.; Kiessling, Laura L.; Hung, Deborah T.; Mecsas, Joan; Aldridge, Bree B.; Isberg, Ralph R.; Blainey, Paul C.
• 署名单位: Massachusetts Institute of Technology (MIT); Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Tufts University; Massachusetts Institute of Technology (MIT); Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Massachusetts Institute of Technology (MIT); Harvard University; Harvard Medical School; Tufts University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9220
• DOI: 10.1073/pnas.2402017122
• 发表日期: 2025-04-01
• 关键词: pseudomonas-aeruginosa; escherichia-coli; chemical screen; permeability; DISCOVERY; strategy; overexpression; antagonism

摘要

Antibiotic resistance, especially in multidrug-resistant ESKAPE pathogens, remains a worldwide problem. Combination antimicrobial therapies may be an important strategy to overcome resistance and broaden the spectrum of existing antibiotics. However, this strategy is limited by the ability to efficiently screen large combinatorial chemical spaces. Here, we deployed a high-throughput combinatorial screening platform, DropArray, to evaluate the interactions of over 30,000 compounds with up to 22 antibiotics and 6 strains of gram-negative ESKAPE pathogens, totaling to over 1.3 million unique strain-antibiotic-compound combinations. In this dataset, compounds more frequently exhibited synergy with known antibiotics than single-agent activity. We identified a compound, P2-56, and developed a more potent analog, P2-56-3, which potentiated rifampin (RIF) against antibiotic-resistant strains of Acinetobacter baumannii and Klebsiella pneumoniae. Using phenotypic assays, we showed P2-56-3 disrupts the outer membrane of A. baumannii. To identify pathways involved in the mechanism of synergy between P2-56-3 and RIF, we performed genetic screens in A. baumannii. CRISPRi-induced partial depletion of lipooligosaccharide transport genes (lptA-D, lptFG) resulted in hypersensitivity to P2-56-3/RIF treatment, demonstrating the genetic dependency of P2-56-3 activity and RIF sensitization on lptgenes in A. baumannii. Consistent with outer membrane homeostasis being an important determinant of P2-56-3/RIF tolerance, knockout of maintenance of lipid asymmetry complex genes and overexpression of certain resistance-nodulation-division efflux pumps-a phenotype associated with multidrug-resistance-resulted in hypersensitivity to P2-56-3. These findings demonstrate the immense scale of phenotypic antibiotic combination screens using DropArray and the potential for such approaches to discover new small molecule synergies against multidrug-resistant ESKAPE strains.