Dynamic forces drive cell and organ morphology changes during embryonic development

• 成果类型: Article
• 署名作者: Manna, Raj Kumar; Retzlaff, Emma M.; Hinman, Anna Maria; Lan, Yiling; Abdel-Razek, Osama; Bates, Mike; Hehnly, Heidi; Amack, Jeffrey D.; Manning, M. Lisa
• 署名单位: Syracuse University; Syracuse University; Indian Institute of Technology System (IIT System); Indian Institute of Technology (IIT) - Kharagpur; State University of New York (SUNY) System; SUNY Upstate Medical University; Syracuse University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8712
• DOI: 10.1073/pnas.2418111122
• 发表日期: 2025-07-22
• 关键词: kupffers vesicle; FLOW; asymmetry; mechanics; symmetry; shapes; brain

摘要

Cells, tissues, and organs must change shape in precise ways during embryonic development to execute their functions. Multiple mechanisms including biochemical signaling pathways and biophysical forces help drive these morphology changes, but it has been difficult to tease apart their contributions, especially from tissue-scale dynamic forces that are typically ignored. We use a combination of mathematical models and in vivo experiments to study a simple organ in the zebrafish embryo called Kupffer's vesicle (KV). Modeling indicates that dynamic forces generated by tissue movements in the embryo produce shape changes in KV that are observed during development. Laser ablations in the zebrafish embryo that alter these forces result in altered organ shapes matching model predictions. These results demonstrate that dynamic forces sculpt cell and organ shape during embryo development.