Compatibility of intracellular binding: Evolutionary design principles for metal sensors

• 成果类型: Article
• 署名作者: Lenner, Nicolas; Chariker, Logan; Leibler, Stanislas
• 署名单位: Institute for Advanced Study - USA; Duke University; Rockefeller University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8485
• DOI: 10.1073/pnas.2427151122
• 发表日期: 2025-05-06
• 关键词: sites; homeostasis

摘要

In the common cellular space, hundreds of binding reactions occur reliably and enable this remarkable compatibility have not yet been adequately elucidated. In order to delineate these principles, we consider the intracellular sensing of transition metals in bacteria-an integral part of cellular metal homeostasis. Protein cytosolic sensors typically interact with metals through three types of lateral chain residues, containing oxygen, nitrogen, or sulfur. The very existence of complete sets of mutually compatible sensors is a nontrivial problem solved by evolution, since each metal sensor has to bind to its cognate metal without being mismetallated by noncognate competitors. Here, based solely on theoretical considerations and limited information about binding transition-metal sensor sets are severely limited in their number by compatibility requirements, leaving only a handful of possible sensor compositions for each transition metal. Our theoretical results turn out to be broadly consistent with experimental data crucial role in the organization and functioning of intracellular processes.