Increased excitatory synapse size in hippocampal place cells compared to silent cells

• 成果类型: Article
• 署名作者: Heredi, Judit; Olah, Gaspar; Sumegi, Mate; Lukacs, Istvan Paul; Aldahabi, Mohammad; Ujfalussy, Balazs B.; Makara, Judit K.; Nusser, Zoltan
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8469
• DOI: 10.1073/pnas.2505322122
• 发表日期: 2025-06-10
• 关键词: statistical structure; ca1 place; plasticity; DYNAMICS; STABILITY; memory; SPACE; time; CODE

摘要

Neuronal activity in the hippocampus creates a cognitive map of space that is essential for navigation. In any given environment, a fraction of hippocampal pyramidal cells (PCs) is active at specific locations (place cells), others are sparsely active without spatial tuning, and a significant proportion of the PCs is entirely silent. The mechanisms underlying the vastly different activities of PCs in the rodent hippocampal CA1 area are unknown. Here, we measured the in vivo activity of CA1 PCs using two-photon [Ca2+] imaging in head-restrained mice during navigation in a virtual corridor and then performed in vitro patch-clamp recording to probe their intrinsic electrical properties and anatomical investigation to characterize their input synapses. The active and passive electrical properties of PCs were similar between PCs with different prior in vivo activities. Perisomatic inhibitory synapse density was also comparable among PCs. The average dendritic spine density and spine head area did not correlate with the mean in vivo activity of PCs, but the size of the spines of place cells was significantly larger compared to that of silent cells. Our results are consistent with excitatory synaptic plasticity as a major mechanism underlying spatially tuned activity of place cells in hippocampal networks.