Multiorgan transcriptomics in mice identifies immunoglobulin heavy constant mu (Ighm) as a tissue-level aging biomarker

• 成果类型: Article
• 署名作者: Yin, Fan-Qian; Wang, Xia-Yan; Li, Yong-Xuan; Li, Kai-Jing; Ma, Si-Yu; Lv, Meng-Jiao; Liu, Zhen-Hua; Zhong, Wei-Xia; Liu, Yan; Tang, Chuan-Fang; Liu, Hong-Shi; Li, Yuze; Xiao, Fu-Hui; Kong, Qing-Peng
• 署名单位: Chinese Academy of Sciences; Kunming Institute of Zoology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Ningbo University; Chinese Academy of Sciences
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8453
• DOI: 10.1073/pnas.2423142122
• 发表日期: 2025-07-15
• 关键词: expression

摘要

Identifying aging-associated biomarkers applicable for multiple tissues is challenging but crucial for assessing tissue aging. Here, we obtained and analyzed 456 transcriptomes on 17 organs from 30 C57BL/6 J mice with different ages, revealing the consistently upregulated mRNAs of Ighm, Cob, and Ccl8 in most aged organs. This finding received support from independent transcriptomic and proteomic datasets and was further validated through western blot, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence, arguing for both Ighm mRNA and protein as tissue-level aging biomarkers, at least in mice. Its sensitivity to antiaging interventions further emphasizes the significance of Ighm in assessing tissue aging in mice.