作者：Lichtenstein, Mauriz A.; Cao, Fakun; Lobnow, Finn; Dirvanskyte, Paulina; Weyhenmeyer, Daniel; Kulesza, Anna; Ziska, Elke; Halfmann, Randal; Taylor, Marcus J.
作者单位：Max Planck Society; University of Kansas; Stowers Institute for Medical Research
摘要：Protein polymer scaffolds composed of death fold (DF) proteins are critical to the formation of signalosomes in immune signaling. The biophysical properties that these polymeric scaffolds require for signal transduction are not clearly defined. Here, we engineered single-component DF signalosomes. We found that functionality depends on the stability provided by the DF polymer, which could also be achieved with a bacterial DF domain, a synthetic filament-forming domain, and amyloid-like sequenc...
作者：Smith, Tara C.
作者单位：University System of Ohio; Kent State University; Kent State University Salem; Kent State University Kent
作者：Zeng, Zhifeng; Hu, Zeyu; Zhao, Ruiliang; Rao, Jikai; Mestre, Mario Rodriguez; Liu, Yanqiu; Liu, Shunhang; Feng, Hao; Chen, Yu; He, Huan; Chen, Nuo; Zheng, Jinshui; Peng, Donghai; Luo, Min; She, Qunxin; Pinilla-Redondo, Rafael; Han, Wenyuan
作者单位：Huazhong Agricultural University; Hubei Hongshan Laboratory; Huazhong Agricultural University; University of Copenhagen; National University of Singapore; Huazhong Agricultural University; Huazhong Agricultural University; Shandong University
摘要：Signaling from pathogen sensing to effector activation is a fundamental principle of cellular immunity. Whereas cyclic (oligo)nucleotides have emerged as key signaling molecules, the existence of other messengers remains largely unexplored. In this study, we reveal a bacterial antiphage system that mediates immune signaling through nucleobase modification. Immunity is triggered by phage nucleotide kinases, which, combined with the system-encoded adenosine deaminase, produce deoxyinosine tripho...
作者：Ghanim, George E.; Hu, Hongmiao; Boulanger, Jerome; Thi Hoang Duong Nguyen
作者单位：MRC Laboratory Molecular Biology
摘要：Long interspersed element-1 (LINE-1) retrotransposons are the only active autonomous transposable elements in humans. They propagate by reverse transcribing their messenger RNA into new genomic locations by a process called target-primed reverse transcription (TPRT). In this work, we present four cryo-electron microscopy structures of the human LINE-1 TPRT complex, revealing the conformational dynamics of open reading frame 2 protein (ORF2p) and its extensive remodeling of the target DNA for T...
