8116期

最新文章

• Molecular basis for methylation-sensitive editing by Cas9 AI翻译
  作者：Roth, Mitchell O.; Shu, Yuerong; Zhao, Yu; Trasanidou, Despoina; Hoffman, Renee D.; Sudfeld, Christian; Bouzetos, Eugenios; Trasanidis, Nikolaos; Zawrotny, Michael; Gelasco, Mary K.; Medina, Megan L.; Das, Anuska; Rai, Jay; Goswami, Hemant N.; Wang, Bing; van der Oost, John; Li, Hong
  作者单位：Van Andel Institute; Wageningen University & Research; Van Andel Institute; Imperial College London; State University System of Florida; Florida State University; State University System of Florida; Florida State University; St Jude Children's Research Hospital; Thermo Fisher Scientific
  摘要：The bacterial CRISPR-Cas9 (Cas9) nuclease has become a powerful genome manipulation tool for a wide range of organisms1, 2-3. However, it has yet to fully leverage the pervasive presence of DNA methylation in genomes4, 5, 6, 7, 8, 9-10. Here, to fill this gap, we report biochemical, structural and human genome-editing characterizations of a methylation-sensitive Cas9 (ThermoCas9). ThermoCas9 efficiently binds to and cleaves DNA upstream of its protospacer adjacent motif (PAM) 5 '-NNNNCGA-3 ' o...
• Are we really headed for a 'super' El Niño? What the science says AI翻译
  作者：Witze, Alexandra
• Could this synthetic egg bring back extinct birds? Researchers urge caution Colossal Biosciences says its artificial egg has de-extinction and conservation potential. AI翻译
  作者：Callaway, Ewen
• Transposable elements are driving rapid adaptation of Enterococcus faecium AI翻译
  作者：Grieshop, Matthew P.; Behr, Aaron A.; Bowden, Sierra; Lin, Jordan D.; Molari, Marco; Reynolds, Gabriella Z. M.; Brooks, Erin F.; Doyle, Boryana; Moore, Ashley A.; Rodriguez-Nava, Guillermo; Salinas, Jorge L.; Banaei, Niaz; Bhatt, Ami S.
  作者单位：Stanford Medicine; Stanford University; Stanford University; Stanford Medicine; Stanford University; Stanford University; Stanford Medicine; Swiss Institute of Bioinformatics; University of Basel; Stanford University; Stanford Medicine; Stanford University; Stanford Medicine; Stanford University; Stanford Medicine; Stanford University; Stanford Medicine; University System of Ohio; Case Western Reserve University
  摘要：Bacterial pathogens adapt rapidly to clinical and within-host selective pressures1. Insertion sequences (IS) are transposable elements that can contribute to pathogenic adaptation2, but their activity and consequences in contemporary clinical populations are not well characterized. Here, combining large-scale genomic surveys with long-read sequencing of clinical isolates and longitudinal gut metagenomes, we quantify pathogen IS dynamics from global patterns to within-host evolution. Across 19,...
• Multiomics and deep learning dissect regulatory syntax in human development AI翻译
  作者：Liu, Betty B.; Jessa, Selin; Kim, Samuel H.; Ng, Yan Ting; Higashino, Soon Il; Marinov, Georgi K.; Chen, Derek C.; Parks, Benjamin E.; Li, Li; Nguyen, Tri C.; Wang, Austin T.; Wang, Sean K.; Tan, Meng How; Tan, Serena Y.; Kosicki, Michael; Pennacchio, Len A.; Ben-David, Eyal; Pasca, Anca M.; Kundaje, Anshul; Farh, Kyle K. H.; Greenleaf, William J.
  作者单位：Stanford University; Stanford University; Stanford Medicine; Stanford University; Stanford University; Illumina; Nanyang Technological University; Stanford University; Stanford Medicine; Stanford University; Lucile Packard Children's Hospital (LPCH); Stanford University; Stanford Medicine; Stanford University; Stanford Medicine; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; United States Department of Energy (DOE); Joint Genome Institute - JGI; University of California System; University of California Berkeley; Stanford University
  摘要：Transcription factors establish cell identity during development by binding regulatory DNA in a sequence-specific manner, often promoting local chromatin accessibility and regulating gene expression1. Mapping accessible chromatin offers critical insights into transcriptional control, but available datasets for human development are restricted to bulk tissue, single organs or single modalities2. Here we present the Human Development Multiomic Atlas, a single-cell atlas of chromatin accessibilit...
• The DNA virome varies with human genes and environments AI翻译
  作者：Kamitaki, Nolan; Tang, David; McCarroll, Steven A.; Loh, Po-Ru
  作者单位：Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Harvard Medical School; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Harvard Medical School; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Harvard Medical School; Harvard University; Howard Hughes Medical Institute; Harvard Medical School
  摘要：Many viruses have adapted to persist in infected humans for life1,2. Variable host control of their ongoing abundance (viral load) can lead to clearance or disease3, 4-5. Here we analysed the viral DNA load of 31 common viruses in human blood and saliva using whole-genome sequencing data from UK Biobank (n = 490,401), All of Us (n = 414,817) and Simons Foundation Powering Autism Research for Knowledge (SPARK; n = 12,519). Viral DNA load varied markedly with age, time of day and season; most vi...
• Spatial atlas of diabetic kidney disease reveals a B cell-rich subgroup AI翻译
  作者：Dumoulin, Bernhard; Levinsohn, Jonathan; Klotzer, Konstantin A.; Li, Chenyu; Mao, Liran; Ha, Eunji; Mohandes, Samer; Nguyen, Thao; Paruzzo, Luca; Hirohama, Daigoro; Fang, Victoria; Bhoj, Vijay G.; Parhiz, Hamideh; Andrade-Silva, Magaiver; Abedini, Amin; Bergeson, Andi; Traum, Daniel; May, Michael J.; Kaestner, Klaus H.; Ruella, Marco; McAllister, Fiona Elizabeth; Hakimi, A. Ari; Li, Mingyao; Palmer, Matthew; Wherry, E. John; Hunter, Christopher A.; Cancro, Michael Paul; Susztak, Katalin
  作者单位：University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; Childrens Hospital of Philadelphia; Medical University of Graz; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; Pennsylvania Medicine; University of Pennsylvania; University of Pennsylvania; Memorial Sloan Kettering Cancer Center; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; Kyungpook National University (KNU); Teikyo University
  摘要：Diabetic kidney disease (DKD), the leading cause of kidney failure, is marked by clinical and molecular heterogeneity, making therapeutic development exceedingly difficult1. Here we used Xenium and CosMx single-cell spatial transcriptomics, integrated with single-nucleus RNA sequencing, to build a cross-platform kidney atlas that makes tissue architecture computable for prognosis, non-invasive detection and patient selection. Using this atlas, we defined reproducible tissue niches and injury-l...
• The Brain, In Theory AI翻译
  作者：Gomez-Marin, Alex
  作者单位：Consejo Superior de Investigaciones Cientificas (CSIC); CSIC-UMH - Instituto de Neurociencias de Alicante (IN); Universidad Miguel Hernandez de Elche; CSIC-UMH - Instituto de Neurociencias de Alicante (IN)
• Iran's Internet blackout: a scholar's month in the dark AI翻译
  作者：Moslehian, Mohammad Sal
  作者单位：Ferdowsi University Mashhad
• Cities affect small and large storms differently AI翻译
  作者：Zhang, Wei
  作者单位：Utah System of Higher Education; Utah State University
  摘要：Analysis of a 23-year record of Texan storms reveals how urban landscapes affect storm rainfall - painting a more complex picture than had been realized.